Time-correlated single molecule localization microscopy enhances resolution and fidelity

Kobi Hermon, Shachar Schidorsky, Yair Razvag, Oren Yakovian, Eilon Sherman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Single-molecule-localization-microscopy (SMLM) enables superresolution imaging of biological samples down to ~ 10–20 nm and in single molecule detail. However, common SMLM reconstruction largely disregards information embedded in the entire intensity trajectories of individual emitters. Here, we develop and demonstrate an approach, termed time-correlated-SMLM (tcSMLM), that uses such information for enhancing SMLM reconstruction. Specifically, tcSMLM is shown to increase the spatial resolution and fidelity of SMLM reconstruction of both simulated and experimental data; esp. upon acquisition under stringent conditions of low SNR, high acquisition rate and high density of emitters. We further provide detailed guidelines and optimization procedures for effectively applying tcSMLM to data of choice. Importantly, our approach can be readily added in tandem to multiple SMLM and related superresolution reconstruction algorithms. Thus, we expect that our approach will become an effective and readily accessible tool for enhancing SMLM and superresolution imaging.

Original languageAmerican English
Article number16212
JournalScientific Reports
Issue number1
StatePublished - 1 Dec 2020

Bibliographical note

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© 2020, The Author(s).


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