TY - JOUR
T1 - Time-resolved emission upon two-photon excitation of bis-N-carbazolyl- distyrylbenzene
T2 - Mapping of water molecule distribution in the mouse brain
AU - Vaganova, Evgenia
AU - Yitzchaik, Shlomo
AU - Sigalov, Mark
AU - Borst, Jan W.
AU - Visser, Antonie
AU - Ovadia, Haim
AU - Khodorkovsky, Vladimir
PY - 2005/8
Y1 - 2005/8
N2 - We present a method of mapping the water molecule distribution in mouse brain tissues using injected bis-N-carbazolyl-distyrylbenzene and the FLIM technique. The fluorescence lifetime of this two-photon absorbing chromophore diminishes when the amount of water in the surrounding area increases. The fluorescence lifetime of the injected in vivo chromophore strongly depends on the content of water in different areas. Thus, lifetimes of 900 ± 50 ps in the hippocampus (extracellular fluid), 520 ± 50 ps in the lateral ventricle (choroid plexus, cerebrospinal fluid), and 400-150 ps in blood vessels were observed. Moreover, the fluorescence lifetime distribution undergoes drastic changes when mice are deprived of water. Statistical analysis of the investigated samples showed that upon water deprivation water content decreased at the border of the hippocampus/lateral ventricle areas and increased in blood vessels.
AB - We present a method of mapping the water molecule distribution in mouse brain tissues using injected bis-N-carbazolyl-distyrylbenzene and the FLIM technique. The fluorescence lifetime of this two-photon absorbing chromophore diminishes when the amount of water in the surrounding area increases. The fluorescence lifetime of the injected in vivo chromophore strongly depends on the content of water in different areas. Thus, lifetimes of 900 ± 50 ps in the hippocampus (extracellular fluid), 520 ± 50 ps in the lateral ventricle (choroid plexus, cerebrospinal fluid), and 400-150 ps in blood vessels were observed. Moreover, the fluorescence lifetime distribution undergoes drastic changes when mice are deprived of water. Statistical analysis of the investigated samples showed that upon water deprivation water content decreased at the border of the hippocampus/lateral ventricle areas and increased in blood vessels.
UR - http://www.scopus.com/inward/record.url?scp=24044518147&partnerID=8YFLogxK
U2 - 10.1039/b418271p
DO - 10.1039/b418271p
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AN - SCOPUS:24044518147
SN - 1144-0546
VL - 29
SP - 1044
EP - 1048
JO - New Journal of Chemistry
JF - New Journal of Chemistry
IS - 8
ER -