Time-resolved low-dose microscopy of glutamine synthetase molecules

W. Kunath*, K. Weiss, H. Sack-Kongehl, M. Kessel, E. Zeitler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Time sequences of low-exposure images from the enzyme molecules of glutamine synthetase have been recorded digitally using an on-line recording system. Processing the images results in a time sequence of averaged molecule images which, in the very beginning, exhibit the molecule structure still undamaged by electron irradiation. Different sources of the noise which obscures structural details, such as shot noise, stain embedding and radiation damage, are investigated.

Original languageEnglish
Pages (from-to)241-252
Number of pages12
JournalUltramicroscopy
Volume13
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

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