Tir tyrosine phosphorylation and pedestal formation are delayed in enteropathogenic Escherichia coli sepZ::TnphoA mutant 30-5-1(3)

R. DeVinney, I. Nisan, S. Ruschkowski, I. Rosenshine, B. B. Finlay*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Enteropathogenic Escherichia coli (EPEC) strain 30-5-1(3) has been reported to form attaching and effacing (A/E) lesions without Tir tyrosine phosphorylation. In this study, we show that 30-5-1(3), which has a transposon insertion within the sepZ gene, forms wild-type A/E lesions including Tir tyrosine phosphorylation, but at a slower rate. A/E lesion formation by 30-5-1(3) occurs without detectable secretion of Tir or other EPEC Esp secreted proteins.

Original languageAmerican English
Pages (from-to)559-563
Number of pages5
JournalInfection and Immunity
Issue number1
StatePublished - 2001
Externally publishedYes

Cite this