Tissue remodeling: A mating-induced differentiation program for the Drosophila oviduct

Anat Kapelnikov, Patricia K. Rivlin, Ronald R. Hoy, Yael Heifetz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Background. In both vertebrates and invertebrates, the oviduct is an epithelial tube surrounded by visceral muscles that serves as a conduit for gamete transport between the ovary and uterus. While Drosophila is a model system for tubular organ development, few studies have addressed the development of the fly's oviduct. Recent studies in Drosophila have identified mating-responsive genes and proteins whose levels in the oviduct are altered by mating. Since many of these molecules (e.g. Muscle LIM protein 84B, Coracle, Neuroglian) have known roles in the differentiation of muscle and epithelia of other organs, mating may trigger similar differentiation events in the oviduct. This led us to hypothesize that mating mediates the last stages of oviduct differentiation in which organ-specific specializations arise. Results. Using electron- and confocal-microscopy we identified tissue-wide post-mating changes in the oviduct including differentiation of cellular junctions, remodeling of extracellular matrix, increased myofibril formation, and increased innervation. Analysis of once- and twice-mated females reveals that some mating-responsive proteins respond only to the first mating, while others respond to both matings. Conclusion. We uncovered ultrastructural changes in the mated oviduct that are consistent with the roles that mating-responsive proteins play in muscle and epithelial differentiation elsewhere. This suggests that mating triggers the late differentiation of the oviduct. Furthermore, we suggest that mating-responsive proteins that respond only to the first mating are involved in the final maturation of the oviduct while proteins that remain responsive to later matings are also involved in maintenance and ongoing function of the oviduct. Taken together, our results establish the oviduct as an attractive system to address mechanisms that regulate the late stages of differentiation and maintenance of a tubular organ.

Original languageAmerican English
Article number114
JournalBMC Developmental Biology
StatePublished - 2008

Bibliographical note

Funding Information:
We thank R. Booker, R. Wyttenbach, I. Carmel and M. Bender for careful reading of this manuscript. We are grateful to Arnon Brand for the female reproductive tract schematic. This research was supported by research grant award 3492 from BARD, The United States-Israel Binational Agricultural Research and Development Fund, Vigevani Fund and Sacker awarded to YH; The Baron de Hirsch Fund Scholarship awarded to AK.


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