@inbook{c9078d3ddb5541889f133798d6ebb9f2,
title = "Topology determination of untagged membrane proteins",
abstract = "The topology of integral membrane proteins with a weak topological tendency can be influenced when fused to tags, such as these used for topological determination or protein purification. Here, we describe a technique for topology determination of an untagged membrane protein. This technique, optimized for bacterial cells, allows the visualization of the protein in the native environment and incorporates the substituted-cysteine accessibility method.",
keywords = "Cysteine, EmrE, Membrane protein, SCAM, Topology, Transport, Untagged",
author = "Iris Nasie and Sonia Steiner-Mordoch and Shimon Schuldiner",
year = "2013",
doi = "10.1007/978-1-62703-487-6_8",
language = "אנגלית",
isbn = "9781627034869",
series = "Methods in Molecular Biology",
pages = "121--130",
editor = "Doron Rapaport and Johannes Herrmann",
booktitle = "Membrane Biogenesis",
}