Topology determination of untagged membrane proteins

Iris Nasie, Sonia Steiner-Mordoch, Shimon Schuldiner

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

13 Scopus citations

Abstract

The topology of integral membrane proteins with a weak topological tendency can be influenced when fused to tags, such as these used for topological determination or protein purification. Here, we describe a technique for topology determination of an untagged membrane protein. This technique, optimized for bacterial cells, allows the visualization of the protein in the native environment and incorporates the substituted-cysteine accessibility method.

Original languageEnglish
Title of host publicationMembrane Biogenesis
Subtitle of host publicationMethods and Protocols
EditorsDoron Rapaport, Johannes Herrmann
Pages121-130
Number of pages10
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1033
ISSN (Print)1064-3745

Keywords

  • Cysteine
  • EmrE
  • Membrane protein
  • SCAM
  • Topology
  • Transport
  • Untagged

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