Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry

Alexander Leitner*, Alexandre M.J.J. Bonvin, Christoph H. Borchers, Robert J. Chalkley, Julia Chamot-Rooke, Colin W. Combe, Jürgen Cox, Meng Qiu Dong, Lutz Fischer, Michael Götze, Fabio C. Gozzo, Albert J.R. Heck, Michael R. Hoopmann, Lan Huang, Yasushi Ishihama, Andrew R. Jones, Nir Kalisman, Oliver Kohlbacher, Karl Mechtler, Robert L. MoritzEugen Netz, Petr Novak, Evgeniy Petrotchenko, Andrej Sali, Richard A. Scheltema, Carla Schmidt, David Schriemer, Andrea Sinz, Frank Sobott, Florian Stengel, Konstantinos Thalassinos, Henning Urlaub, Rosa Viner, Juan A. Vizcaíno, Marc R. Wilkins, Juri Rappsilber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative.

Original languageAmerican English
Pages (from-to)1259-1268
Number of pages10
Issue number11
StatePublished - 3 Nov 2020

Bibliographical note

Publisher Copyright:
© 2020


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