Abstract
Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative.
Original language | American English |
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Pages (from-to) | 1259-1268 |
Number of pages | 10 |
Journal | Structure |
Volume | 28 |
Issue number | 11 |
DOIs | |
State | Published - 3 Nov 2020 |
Bibliographical note
Funding Information:We thank the participants of the meetings mentioned throughout the text for their many different contributions. Although we made an effort to include all labs that may feel a belonging to the field of Cross-linking MS, we apologize to all those that we missed. Developing standards in Cross-linking MS is to be viewed as an inclusive activity, so please feel warmly invited to join our efforts. This work was supported by the Wellcome Trust through a Senior Research Fellowship to J.R. ( 103139 ) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, project 329673113 ). Funded by the DFG under Germany's Excellence Strategy, EXC 2008/1- 390540038 . The Wellcome Centre for Cell Biology is supported by core funding from the Wellcome Trust ( 203149 ).
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© 2020