Towards enhancing therapeutic glycoprotein bioproduction: Interventions in the PI3K/AKT/mTOR pathway

Mohamed Mahameed; Afnan Sulieman; Duah Alkam; Boaz Tirosh

doi:10.1247/csf.19013

Towards enhancing therapeutic glycoprotein bioproduction: Interventions in the PI3K/AKT/mTOR pathway

Mohamed Mahameed, Afnan Sulieman, Duah Alkam, Boaz Tirosh^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Short survey › peer-review

3 Scopus citations

Abstract

Recombinant glycoproteins produced in mammalian cells are clinically indispensable drugs used to treat a broad spectrum of diseases. Their bio-manufacturing process is laborious, time consuming, and expensive. Investment in expediting the process and reducing its cost is the subject of continued research. The PI3K/Akt/ mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. In this review we detail various strategies previously employed to increase glycoprotein production yields via either genetic or pharmacological over-activation of the PI3K/Akt/mTOR pathway, and we discuss their potential and limitations.

Original language	English
Pages (from-to)	75-83
Number of pages	9
Journal	Cell Structure and Function
Volume	44
Issue number	2
DOIs	https://doi.org/10.1247/csf.19013
State	Published - 2019

Bibliographical note

Publisher Copyright:
© 2019 The Author(s).

Keywords

CRISPR
MTORC1
Specific productivity
Translation

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10.1247/csf.19013

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abstract = "Recombinant glycoproteins produced in mammalian cells are clinically indispensable drugs used to treat a broad spectrum of diseases. Their bio-manufacturing process is laborious, time consuming, and expensive. Investment in expediting the process and reducing its cost is the subject of continued research. The PI3K/Akt/ mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. In this review we detail various strategies previously employed to increase glycoprotein production yields via either genetic or pharmacological over-activation of the PI3K/Akt/mTOR pathway, and we discuss their potential and limitations.",

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AU - Alkam, Duah

AU - Tirosh, Boaz

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AB - Recombinant glycoproteins produced in mammalian cells are clinically indispensable drugs used to treat a broad spectrum of diseases. Their bio-manufacturing process is laborious, time consuming, and expensive. Investment in expediting the process and reducing its cost is the subject of continued research. The PI3K/Akt/ mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. In this review we detail various strategies previously employed to increase glycoprotein production yields via either genetic or pharmacological over-activation of the PI3K/Akt/mTOR pathway, and we discuss their potential and limitations.

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KW - Specific productivity

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Towards enhancing therapeutic glycoprotein bioproduction: Interventions in the PI3K/AKT/mTOR pathway

Abstract

Bibliographical note

Keywords

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