Towards the first linkage map of the Didymella rabiei genome

J. Lichtenzveig; P. Winter; S. Abbo; D. Shtienberg; W. J. Kaiser; G. Kahl

doi:10.1007/BF02979751

Towards the first linkage map of the Didymella rabiei genome

J. Lichtenzveig, P. Winter, S. Abbo, D. Shtienberg, W. J. Kaiser, G. Kahl

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F₁ progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ² <0.05). The implications of our results on ploidy level of the asexual spores are discussed.

Original language	English
Pages (from-to)	467-472
Number of pages	6
Journal	Phytoparasitica
Volume	30
Issue number	5
DOIs	https://doi.org/10.1007/BF02979751
State	Published - 2002

Bibliographical note

Funding Information:
J. Lichtenzveig acknowledges fellowship support from the Deutscher Akademischer Austauschdienst (DAAD) and UNESCO (Paris, France). This project was supported by a grant from BMZ, Germany (No. 89.7860-01.130). The authors thank anonymous reviewers for their comments.

Keywords

Ascochyta blight
Chickpea
DAF
Linkage map
Molecular markers
Ploidy level
STMS

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10.1007/BF02979751

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title = "Towards the first linkage map of the Didymella rabiei genome",

abstract = "A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F1 progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ2 <0.05). The implications of our results on ploidy level of the asexual spores are discussed.",

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author = "J. Lichtenzveig and P. Winter and S. Abbo and D. Shtienberg and Kaiser, {W. J.} and G. Kahl",

note = "Funding Information: J. Lichtenzveig acknowledges fellowship support from the Deutscher Akademischer Austauschdienst (DAAD) and UNESCO (Paris, France). This project was supported by a grant from BMZ, Germany (No. 89.7860-01.130). The authors thank anonymous reviewers for their comments.",

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AU - Winter, P.

AU - Abbo, S.

AU - Shtienberg, D.

AU - Kaiser, W. J.

AU - Kahl, G.

N1 - Funding Information: J. Lichtenzveig acknowledges fellowship support from the Deutscher Akademischer Austauschdienst (DAAD) and UNESCO (Paris, France). This project was supported by a grant from BMZ, Germany (No. 89.7860-01.130). The authors thank anonymous reviewers for their comments.

PY - 2002

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N2 - A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F1 progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ2 <0.05). The implications of our results on ploidy level of the asexual spores are discussed.

AB - A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F1 progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ2 <0.05). The implications of our results on ploidy level of the asexual spores are discussed.

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KW - Ploidy level

KW - STMS

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Towards the first linkage map of the Didymella rabiei genome

Abstract

Bibliographical note

Keywords

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