Tracking GLUT2 translocation by live-cell imaging

Sabina Tsytkin-Kirschenzweig; Merav Cohen; Yaakov Nahmias

doi:10.1007/978-1-4939-7507-5_18

Tracking GLUT2 translocation by live-cell imaging

Sabina Tsytkin-Kirschenzweig, Merav Cohen, Yaakov Nahmias^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

The facilitative glucose transporter (GLUT) family plays a key role in metabolic homeostasis, controlling the absorption rates and rapid response to changing carbohydrate levels. The facilitative GLUT2 transporter is uniquely expressed in metabolic epithelial cells of the intestine, pancreas, liver, and kidney. GLUT2 dysfunction is associated with several pathologies, including Fanconi-Bickel syndrome, a glycogen storage disease, characterized by growth retardation and renal dysfunction. Interestingly, GLUT2 activity is modulated by its cellular localization. Membrane translocation specifically regulates GLUT2 activity in enterocytes, pancreatic β-cells, hepatocytes, and proximal tubule cells. We have established a system to visualize and quantify GLUT2 translocation, and its dynamics, by live imaging of a mCherry-hGLUT2 fusion protein in polarized epithelial cells. This system enables testing of putative modulators of GLUT2 translocation, which are potential drugs for conditions of impaired glucose homeostasis and associated nephropathy.

Original language	American English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	241-254
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-7507-5_18
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1713
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work was supported by the European Research Council Consolidator Grant (OCLD 681870), and through the generous gift of Sam and Rina Frankel. Resources were provided by the Silberman Institute of Life Sciences and the Alexander Grass Center for Bioengineering of the Hebrew University of Jerusalem.

Publisher Copyright:
© Springer Science+Business Media LLC 2018.

Keywords

GLUT2
Glucose transport
Kidney function
Live imaging
Polarized epithelium
Translocation
Type 2 diabetes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-7507-5_18

Cite this

@inbook{d4b698733428412aa0aa76bb27d007fc,

title = "Tracking GLUT2 translocation by live-cell imaging",

abstract = "The facilitative glucose transporter (GLUT) family plays a key role in metabolic homeostasis, controlling the absorption rates and rapid response to changing carbohydrate levels. The facilitative GLUT2 transporter is uniquely expressed in metabolic epithelial cells of the intestine, pancreas, liver, and kidney. GLUT2 dysfunction is associated with several pathologies, including Fanconi-Bickel syndrome, a glycogen storage disease, characterized by growth retardation and renal dysfunction. Interestingly, GLUT2 activity is modulated by its cellular localization. Membrane translocation specifically regulates GLUT2 activity in enterocytes, pancreatic β-cells, hepatocytes, and proximal tubule cells. We have established a system to visualize and quantify GLUT2 translocation, and its dynamics, by live imaging of a mCherry-hGLUT2 fusion protein in polarized epithelial cells. This system enables testing of putative modulators of GLUT2 translocation, which are potential drugs for conditions of impaired glucose homeostasis and associated nephropathy.",

keywords = "GLUT2, Glucose transport, Kidney function, Live imaging, Polarized epithelium, Translocation, Type 2 diabetes",

author = "Sabina Tsytkin-Kirschenzweig and Merav Cohen and Yaakov Nahmias",

note = "Funding Information: This work was supported by the European Research Council Consolidator Grant (OCLD 681870), and through the generous gift of Sam and Rina Frankel. Resources were provided by the Silberman Institute of Life Sciences and the Alexander Grass Center for Bioengineering of the Hebrew University of Jerusalem. Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2018.",

year = "2018",

doi = "10.1007/978-1-4939-7507-5_18",

language = "American English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "241--254",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Tracking GLUT2 translocation by live-cell imaging

AU - Tsytkin-Kirschenzweig, Sabina

AU - Cohen, Merav

AU - Nahmias, Yaakov

N1 - Funding Information: This work was supported by the European Research Council Consolidator Grant (OCLD 681870), and through the generous gift of Sam and Rina Frankel. Resources were provided by the Silberman Institute of Life Sciences and the Alexander Grass Center for Bioengineering of the Hebrew University of Jerusalem. Publisher Copyright: © Springer Science+Business Media LLC 2018.

PY - 2018

Y1 - 2018

N2 - The facilitative glucose transporter (GLUT) family plays a key role in metabolic homeostasis, controlling the absorption rates and rapid response to changing carbohydrate levels. The facilitative GLUT2 transporter is uniquely expressed in metabolic epithelial cells of the intestine, pancreas, liver, and kidney. GLUT2 dysfunction is associated with several pathologies, including Fanconi-Bickel syndrome, a glycogen storage disease, characterized by growth retardation and renal dysfunction. Interestingly, GLUT2 activity is modulated by its cellular localization. Membrane translocation specifically regulates GLUT2 activity in enterocytes, pancreatic β-cells, hepatocytes, and proximal tubule cells. We have established a system to visualize and quantify GLUT2 translocation, and its dynamics, by live imaging of a mCherry-hGLUT2 fusion protein in polarized epithelial cells. This system enables testing of putative modulators of GLUT2 translocation, which are potential drugs for conditions of impaired glucose homeostasis and associated nephropathy.

AB - The facilitative glucose transporter (GLUT) family plays a key role in metabolic homeostasis, controlling the absorption rates and rapid response to changing carbohydrate levels. The facilitative GLUT2 transporter is uniquely expressed in metabolic epithelial cells of the intestine, pancreas, liver, and kidney. GLUT2 dysfunction is associated with several pathologies, including Fanconi-Bickel syndrome, a glycogen storage disease, characterized by growth retardation and renal dysfunction. Interestingly, GLUT2 activity is modulated by its cellular localization. Membrane translocation specifically regulates GLUT2 activity in enterocytes, pancreatic β-cells, hepatocytes, and proximal tubule cells. We have established a system to visualize and quantify GLUT2 translocation, and its dynamics, by live imaging of a mCherry-hGLUT2 fusion protein in polarized epithelial cells. This system enables testing of putative modulators of GLUT2 translocation, which are potential drugs for conditions of impaired glucose homeostasis and associated nephropathy.

KW - GLUT2

KW - Glucose transport

KW - Kidney function

KW - Live imaging

KW - Polarized epithelium

KW - Translocation

KW - Type 2 diabetes

UR - http://www.scopus.com/inward/record.url?scp=85037585536&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7507-5_18

DO - 10.1007/978-1-4939-7507-5_18

M3 - Chapter

C2 - 29218530

AN - SCOPUS:85037585536

T3 - Methods in Molecular Biology

SP - 241

EP - 254

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Tracking GLUT2 translocation by live-cell imaging

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this