TY - JOUR
T1 - Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles
AU - Portnoy, Emma
AU - Polyak, Boris
AU - Inbar, Dorrit
AU - Kenan, Gilad
AU - Rai, Ahmad
AU - Wehrli, Suzanne L.
AU - Roberts, Timothy P.L.
AU - Bishara, Ameer
AU - Mann, Aniv
AU - Shmuel, Miriam
AU - Rozovsky, Katya
AU - Itzhak, Gal
AU - Ben-Hur, Tamir
AU - Magdassi, Shlomo
AU - Ekstein, Dana
AU - Eyal, Sara
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy.
AB - Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy.
KW - Epilepsy
KW - Imaging
KW - Inflammation
KW - Magnetic Resonance Imaging
KW - Magnetic nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84963642800&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2016.01.018
DO - 10.1016/j.nano.2016.01.018
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 26964483
AN - SCOPUS:84963642800
SN - 1549-9634
VL - 12
SP - 1335
EP - 1345
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 5
ER -