TY - JOUR
T1 - Transcription of DNA and chromatin with calf thymus RNA polymerase B in vitro
AU - Cedar, Howard
PY - 1975/6/25
Y1 - 1975/6/25
N2 - The interaction of calf thymus RNA polymerase B with DNA and chromatin has been studied under conditions which permit direct measurement of binding sites capable of supporting chain initiation. The number of such sites is determined by counting the number of growing RNA chains, using either sucrose gradient ultracentrifugation or incorporation of [γ-32P]ATP and [γ32P]GTP. These measurements show that there is one site per 40,000 nucleotide pairs on DNA and one-tenth that number on chromatin. This is in contrast to the bacterial RNA polymerase, which has many more initiation sites both on DNA and on chromatin.
AB - The interaction of calf thymus RNA polymerase B with DNA and chromatin has been studied under conditions which permit direct measurement of binding sites capable of supporting chain initiation. The number of such sites is determined by counting the number of growing RNA chains, using either sucrose gradient ultracentrifugation or incorporation of [γ-32P]ATP and [γ32P]GTP. These measurements show that there is one site per 40,000 nucleotide pairs on DNA and one-tenth that number on chromatin. This is in contrast to the bacterial RNA polymerase, which has many more initiation sites both on DNA and on chromatin.
UR - http://www.scopus.com/inward/record.url?scp=0016814664&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(75)90394-0
DO - 10.1016/0022-2836(75)90394-0
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C2 - 1102705
AN - SCOPUS:0016814664
SN - 0022-2836
VL - 95
SP - 257
EP - 269
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -