Transcriptional profiling and localization of GUL-1, a COT-1 pathway component, in Neurospora crassa

Inbal Herold, David Kowbel, Diego L. Delgado-Álvarez, Marisela Garduño-Rosales, Rosa R. Mouriño-Pérez, Oded Yarden*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Impairment of the Neurospora crassa COT-1 kinase results in defects in hyphal polarity. Some of these effects are partially suppressed by inactivation of gul-1 (encoding an mRNA-binding protein involved in translational regulation). Here, we report on the transcriptional profiling of cot-1 inactivation and demonstrate that gul-1 affects transcript abundance of multiple genes in the COT-1 pathway, including processes such as cell wall remodeling, nitrogen and amino acid metabolism. The GUL-1 protein itself was found to be distributed within the entire hyphal cell, along with a clear presence of aggregates that traffic within the cytoplasm. Live imaging of GUL-1-GFP demonstrated that GUL-1 transport is microtubule-dependent. Cellular stress, as imposed by the presence of the cell wall biosynthesis inhibitor Nikkomycin Z or by nitrogen limitation, resulted in a 2–3-fold increase of GUL-1 aggregate association with nuclei. Taken together, this study demonstrates that GUL-1 affects multiple processes, its function is stress-related and linked with cellular traffic and nuclear association.

Original languageAmerican English
Pages (from-to)1-11
Number of pages11
JournalFungal Genetics and Biology
Volume126
DOIs
StatePublished - May 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.

Fingerprint

Dive into the research topics of 'Transcriptional profiling and localization of GUL-1, a COT-1 pathway component, in Neurospora crassa'. Together they form a unique fingerprint.

Cite this