Abstract
Microsomal triglyceride transfer protein (MTP) catalyzes the assembly of triglyceride (TG)-rich apolipoprotein B-containing liver (e.g., VLDL) and intestinal (e.g., chylomicron) lipoproteins. The human MTP gene promoter is reported here to associate in vivo with endogenous hepatocyte nuclear factor-4α (HNF-4α) and to be transactivated or transsuppressed by overexpressed or by dominant negative HNF-4α, respectively. Human MTP (hMTP) transactivation by HNF-4α is accounted for by the concerted activity of distal (-83/-70) and proximal (-50/-38) direct repeat 1 elements of the hMTP promoter that bind HNF-4α. Transactivation by HNF-4α is specifically antagonized by chicken ovalbumin upstream promoter. Transcriptional activation of hMTP by HNF-4α is mediated by HNF-4α domains engaged in ligand binding and ligand-driven transactivation and is further complemented by HNF-4α/HNF-1α synergism that involves the HNF-4α activation function 1 (AF-1) domain. hMTP transactivation by HNF-4α is specifically inhibited by β,β-tetramethyl-hexadecanedioic acid acting as an HNF-4α antagonist ligand. hMTP transactivation by HNF-4α may account for the activation or inhibition of MTP expression and the production of TG-rich lipoproteins by agonist (e.g., saturated fatty acids) or antagonist [e.g., (n-3) PUFA, hypolipidemic fibrates, or Methyl-substituted dicarboxylic acid (Medica) compounds] HNF-4α ligands.
Original language | American English |
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Pages (from-to) | 328-341 |
Number of pages | 14 |
Journal | Journal of Lipid Research |
Volume | 46 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2005 |
Keywords
- Hypolipidemic drugs
- Lipoproteins
- Medica 16
- Nuclear receptors