Transcriptome analysis highlights nuclear control of chloroplast development in the shoot apex

Vijay Dalal, Shlomi Dagan, Gilgi Friedlander, Elinor Aviv, Ralph Bock, Dana Charuvi, Ziv Reich*, Zach Adam

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


In dicots, the key developmental process by which immature plastids differentiate into photosynthetically competent chloroplasts commences in the shoot apical meristem (SAM), within the shoot apex. Using laser-capture microdissection and single-cell RNA sequencing methodology, we studied the changes in the transcriptome along the chloroplast developmental pathway in the shoot apex of tomato seedlings. The analysis revealed the presence of transcripts for different chloroplast functions already in the stem cell-containing region of the SAM. Thereafter, an en masse up-regulation of genes encoding for various proteins occurs, including chloroplast ribosomal proteins and proteins involved in photosynthesis, photoprotection and detoxification of reactive oxygen species. The results highlight transcriptional events that operate during chloroplast biogenesis, leading to the rapid establishment of photosynthetic competence.

Original languageAmerican English
Article number8881
JournalScientific Reports
Issue number1
StatePublished - 1 Dec 2018

Bibliographical note

Funding Information:
This work was supported by a postdoctoral fellowship from The PBC Program of the Council for Higher Education to V.D., grants from the Israel Science Foundation (1034/12), and National Science Foundation-United States-Israel Binational Science Foundation (NSF-BSF) Molecular and Cellular Biosciences Program (2015839) to Z.R., the Israel Science Foundation (121/13) and the Israel Ministry of Agriculture (837-0123-12) to Z.A., and a joint grant from the Human Frontier Science Program (RGP0005/2013) to R.B. and Z.R. We thank Dr. Tal Katz-Ezov and Olga Karinski for their invaluable help with the RNA sequencing procedures.

Publisher Copyright:
© 2018 The Author(s).


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