Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding

Nena Pavlidi; Anastasia Gioti; Nicky Wybouw; Wannes Dermauw; Michael Ben-Yosef; Boaz Yuval; Edouard Jurkevich; Anastasia Kampouraki; Thomas Van Leeuwen; John Vontas

doi:10.1038/srep42633

Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding

Nena Pavlidi, Anastasia Gioti, Nicky Wybouw, Wannes Dermauw, Michael Ben-Yosef, Boaz Yuval, Edouard Jurkevich, Anastasia Kampouraki, Thomas Van Leeuwen, John Vontas^*

^*Corresponding author for this work

Department of Entomology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The olive fruit fly, Bactrocera oleae, is the most destructive pest of olive orchards worldwide. The monophagous larva has the unique capability of feeding on olive mesocarp, coping with high levels of phenolic compounds and utilizing non-hydrolyzed proteins present, particularly in the unripe, green olives. On the molecular level, the interaction between B. oleae and olives has not been investigated as yet. Nevertheless, it has been associated with the gut obligate symbiotic bacterium Candidatus Erwinia dacicola. Here, we used a B.oleae microarray to analyze the gene expression of larvae during their development in artificial diet, unripe (green) and ripe (black) olives. The expression profiles of Ca. E. dacicola were analyzed in parallel, using the Illumina platform. Several genes were found overexpressed in the olive fly larvae when feeding in green olives. Among these, a number of genes encoding detoxification and digestive enzymes, indicating a potential association with the ability of B. oleae to cope with green olives. In addition, a number of biological processes seem to be activated in Ca. E. dacicola during the development of larvae in olives, with the most notable being the activation of amino-acid metabolism.

Original language	American English
Article number	42633
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/srep42633
State	Published - 22 Feb 2017

Bibliographical note

Funding Information:
NP was supported by the European Commission (EC contract 618105) via FACCE ERA-NET Plus and FACCE-JP (Genomite, project ID 137). NW was supported by a Marie Sklodowska-Curie Action (MSCA) Individual fellowship (658795-DOGMITE) of Horizon 2020. WD is a postdoctoral fellow of the Fund for Scientific Research Flanders (FWO). This project was supported by the Fund for Scientific Research Flanders (FWO) (grant G053815N to TVL and WD). AG, NP and JV were supported by the project "Genomic approaches for understanding xenobiotic detoxification of the olive fruit fly and its adaptation to olives, and biological genetic sexing for improving SIT" and code number 4937, implemented under the "ARISTEIA" Action of the "OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING" and co-funded by the European Social Fund (ESF) and National Resources.

Publisher Copyright:
© 2017 The Author(s).

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@article{ea046134a50343288c54c7adca2ba80e,

title = "Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding",

abstract = "The olive fruit fly, Bactrocera oleae, is the most destructive pest of olive orchards worldwide. The monophagous larva has the unique capability of feeding on olive mesocarp, coping with high levels of phenolic compounds and utilizing non-hydrolyzed proteins present, particularly in the unripe, green olives. On the molecular level, the interaction between B. oleae and olives has not been investigated as yet. Nevertheless, it has been associated with the gut obligate symbiotic bacterium Candidatus Erwinia dacicola. Here, we used a B.oleae microarray to analyze the gene expression of larvae during their development in artificial diet, unripe (green) and ripe (black) olives. The expression profiles of Ca. E. dacicola were analyzed in parallel, using the Illumina platform. Several genes were found overexpressed in the olive fly larvae when feeding in green olives. Among these, a number of genes encoding detoxification and digestive enzymes, indicating a potential association with the ability of B. oleae to cope with green olives. In addition, a number of biological processes seem to be activated in Ca. E. dacicola during the development of larvae in olives, with the most notable being the activation of amino-acid metabolism.",

author = "Nena Pavlidi and Anastasia Gioti and Nicky Wybouw and Wannes Dermauw and Michael Ben-Yosef and Boaz Yuval and Edouard Jurkevich and Anastasia Kampouraki and {Van Leeuwen}, Thomas and John Vontas",

note = "Funding Information: NP was supported by the European Commission (EC contract 618105) via FACCE ERA-NET Plus and FACCE-JP (Genomite, project ID 137). NW was supported by a Marie Sklodowska-Curie Action (MSCA) Individual fellowship (658795-DOGMITE) of Horizon 2020. WD is a postdoctoral fellow of the Fund for Scientific Research Flanders (FWO). This project was supported by the Fund for Scientific Research Flanders (FWO) (grant G053815N to TVL and WD). AG, NP and JV were supported by the project {"}Genomic approaches for understanding xenobiotic detoxification of the olive fruit fly and its adaptation to olives, and biological genetic sexing for improving SIT{"} and code number 4937, implemented under the {"}ARISTEIA{"} Action of the {"}OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING{"} and co-funded by the European Social Fund (ESF) and National Resources. Publisher Copyright: {\textcopyright} 2017 The Author(s).",

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doi = "10.1038/srep42633",

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AU - Pavlidi, Nena

AU - Gioti, Anastasia

AU - Wybouw, Nicky

AU - Dermauw, Wannes

AU - Ben-Yosef, Michael

AU - Yuval, Boaz

AU - Jurkevich, Edouard

AU - Kampouraki, Anastasia

AU - Van Leeuwen, Thomas

AU - Vontas, John

N1 - Funding Information: NP was supported by the European Commission (EC contract 618105) via FACCE ERA-NET Plus and FACCE-JP (Genomite, project ID 137). NW was supported by a Marie Sklodowska-Curie Action (MSCA) Individual fellowship (658795-DOGMITE) of Horizon 2020. WD is a postdoctoral fellow of the Fund for Scientific Research Flanders (FWO). This project was supported by the Fund for Scientific Research Flanders (FWO) (grant G053815N to TVL and WD). AG, NP and JV were supported by the project "Genomic approaches for understanding xenobiotic detoxification of the olive fruit fly and its adaptation to olives, and biological genetic sexing for improving SIT" and code number 4937, implemented under the "ARISTEIA" Action of the "OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING" and co-funded by the European Social Fund (ESF) and National Resources. Publisher Copyright: © 2017 The Author(s).

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N2 - The olive fruit fly, Bactrocera oleae, is the most destructive pest of olive orchards worldwide. The monophagous larva has the unique capability of feeding on olive mesocarp, coping with high levels of phenolic compounds and utilizing non-hydrolyzed proteins present, particularly in the unripe, green olives. On the molecular level, the interaction between B. oleae and olives has not been investigated as yet. Nevertheless, it has been associated with the gut obligate symbiotic bacterium Candidatus Erwinia dacicola. Here, we used a B.oleae microarray to analyze the gene expression of larvae during their development in artificial diet, unripe (green) and ripe (black) olives. The expression profiles of Ca. E. dacicola were analyzed in parallel, using the Illumina platform. Several genes were found overexpressed in the olive fly larvae when feeding in green olives. Among these, a number of genes encoding detoxification and digestive enzymes, indicating a potential association with the ability of B. oleae to cope with green olives. In addition, a number of biological processes seem to be activated in Ca. E. dacicola during the development of larvae in olives, with the most notable being the activation of amino-acid metabolism.

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Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding

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