TY - JOUR
T1 - Transcutaneous immunization with hydrophilic recombinant gp100 protein induces antigen-specific cellular immune response
AU - Eisenberg, Galit
AU - Machlenkin, Arthur
AU - Frankenburg, Shoshana
AU - Mansura, Adva
AU - Pitcovski, Jacob
AU - Yefenof, Eitan
AU - Peretz, Tamar
AU - Lotem, Michal
PY - 2010
Y1 - 2010
N2 - The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate that HR-gp100 permeates into human skin, and is processed and presented by human dendritic cells. In a mouse model, an HR-gp100-based vaccine triggered antigen-specific T cell responses, as shown by proliferation assays, ELISA and intracellular staining for IFN-γ. Transcutaneous antigen delivery may provide a safe, simple and effective method to elicit cell-mediated immunity.
AB - The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate that HR-gp100 permeates into human skin, and is processed and presented by human dendritic cells. In a mouse model, an HR-gp100-based vaccine triggered antigen-specific T cell responses, as shown by proliferation assays, ELISA and intracellular staining for IFN-γ. Transcutaneous antigen delivery may provide a safe, simple and effective method to elicit cell-mediated immunity.
KW - Cancer vaccine
KW - Gp100
KW - Transcutaneous immunization
UR - http://www.scopus.com/inward/record.url?scp=77958489663&partnerID=8YFLogxK
U2 - 10.1016/j.cellimm.2010.09.003
DO - 10.1016/j.cellimm.2010.09.003
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C2 - 20947070
AN - SCOPUS:77958489663
SN - 0008-8749
VL - 266
SP - 98
EP - 103
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -