Transcytosis of Streptococcus iniae through skin epithelial barriers: An in vitro study

Marina Eyngor, Stefan Chilmonczyk, Amir Zlotkin, Elisabetta Manuali, Dan Lahav, Claudio Ghittino, Roni Shapira, Avshalom Hurvitz, Avi Eldar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


By constructing a biological model based on in vitro culture of polarized rainbow trout primary skin epithelial cell monolayers, the series of early events that precede Streptococcus iniae infection, particularly colonization and translocation through external barriers, were analyzed. Streptococcus iniae promptly invades skin epithelial cells, but the rapid decline of viable intracellular bacteria points out the limited capability of intracellular survival for this bacterium. Translocation assays, supported by electron microscopy microphotographs, demonstrate that following successful in vitro invasion of skin epithelial cell, the bacterium exists free in the cytoplasm after release from the endosome, and translocates through the skin barrier. Bacterial invasion and transcytosis is not accompanied by apparent cell-line damages or disruption of host cells' tight junctions. It is hypothesized that the phenomenon of epithelial invasion coupled to the rapid translocation through the barrier plays a crucial role in Streptococcus iniae infection.

Original languageAmerican English
Pages (from-to)238-248
Number of pages11
JournalFEMS Microbiology Letters
Issue number2
StatePublished - Dec 2007


  • Adhesion
  • Invasion
  • Skin
  • Streptococcus iniae
  • Translocation


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