TY - JOUR
T1 - Transformation of cultured human embryonic fibroblasts by oncornavirus like particles released from a human carcinoma cell line
AU - Balabanova, H.
AU - Kotler, M.
AU - Becker, Y.
PY - 1975
Y1 - 1975
N2 - A fibroblast like cell culture was established from a stomach biopsy of a patient with metastatic adenocarcinoma. One of the cultures, at the 6th passage level, left unattended for a month at 37°, produced numerous foci of epithelial cells. Upon subculturing, an epithelial cell line, designated HCCL (human carcinoma cell line), was established. The HCCL cells released particles possessing the characteristics of oncornaviruses: density 1.175 g/ml, cores with a density of 1.22-1.26 g/ml, high molecular weight RNA (60-70S) and RNA instructed DNA polymerase activity (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). Inoculation of particles released from HCCL cells into cultures of human embryo muscle fibroblasts resulted in the appearance of foci of transformed cells.
AB - A fibroblast like cell culture was established from a stomach biopsy of a patient with metastatic adenocarcinoma. One of the cultures, at the 6th passage level, left unattended for a month at 37°, produced numerous foci of epithelial cells. Upon subculturing, an epithelial cell line, designated HCCL (human carcinoma cell line), was established. The HCCL cells released particles possessing the characteristics of oncornaviruses: density 1.175 g/ml, cores with a density of 1.22-1.26 g/ml, high molecular weight RNA (60-70S) and RNA instructed DNA polymerase activity (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). Inoculation of particles released from HCCL cells into cultures of human embryo muscle fibroblasts resulted in the appearance of foci of transformed cells.
UR - http://www.scopus.com/inward/record.url?scp=0016764404&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.7.2794
DO - 10.1073/pnas.72.7.2794
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C2 - 52157
AN - SCOPUS:0016764404
SN - 0027-8424
VL - 72
SP - 2794
EP - 2798
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -
