Transgenic Nicotiana benthamiana and grapevine plants transformed with grapevine virus A (GVA) sequences

S. Radian-Sade; A. Perl; Orit Edelbaum; Larissa Kuznetsova; R. Gafny; I. Sela; Edna Tanne

doi:10.1007/BF02994025

Transgenic Nicotiana benthamiana and grapevine plants transformed with grapevine virus A (GVA) sequences

S. Radian-Sade^*, A. Perl, Orit Edelbaum, Larissa Kuznetsova, R. Gafny, I. Sela, Edna Tanne

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

As part of a project to construct virus-resistant transgenic grapevines, grapevine virus A (GVA) coat protein (CP) sequences were introduced into grapevine and the model plant Nicotiana benthamiana. The engineered constructs were tested for their ability to engender resistance in N. benthamiana. A number of GVA-CP-transformed N. benthamiana lines have become resistant to GVA. Preliminary biological and molecular analyses strongly suggest that in most cases the obtained resistance is RNA-mediated. Several lines of transgenic grapevine were obtained.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Phytoparasitica
Volume	28
Issue number	1
DOIs	https://doi.org/10.1007/BF02994025
State	Published - 2000

Keywords

Coat protein
Grapevine
Transgenic plants
Virus resistance
Vitivirus

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10.1007/BF02994025

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title = "Transgenic Nicotiana benthamiana and grapevine plants transformed with grapevine virus A (GVA) sequences",

abstract = "As part of a project to construct virus-resistant transgenic grapevines, grapevine virus A (GVA) coat protein (CP) sequences were introduced into grapevine and the model plant Nicotiana benthamiana. The engineered constructs were tested for their ability to engender resistance in N. benthamiana. A number of GVA-CP-transformed N. benthamiana lines have become resistant to GVA. Preliminary biological and molecular analyses strongly suggest that in most cases the obtained resistance is RNA-mediated. Several lines of transgenic grapevine were obtained.",

keywords = "Coat protein, Grapevine, Transgenic plants, Virus resistance, Vitivirus",

author = "S. Radian-Sade and A. Perl and Orit Edelbaum and Larissa Kuznetsova and R. Gafny and I. Sela and Edna Tanne",

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T1 - Transgenic Nicotiana benthamiana and grapevine plants transformed with grapevine virus A (GVA) sequences

AU - Radian-Sade, S.

AU - Perl, A.

AU - Edelbaum, Orit

AU - Kuznetsova, Larissa

AU - Gafny, R.

AU - Sela, I.

AU - Tanne, Edna

PY - 2000

Y1 - 2000

N2 - As part of a project to construct virus-resistant transgenic grapevines, grapevine virus A (GVA) coat protein (CP) sequences were introduced into grapevine and the model plant Nicotiana benthamiana. The engineered constructs were tested for their ability to engender resistance in N. benthamiana. A number of GVA-CP-transformed N. benthamiana lines have become resistant to GVA. Preliminary biological and molecular analyses strongly suggest that in most cases the obtained resistance is RNA-mediated. Several lines of transgenic grapevine were obtained.

AB - As part of a project to construct virus-resistant transgenic grapevines, grapevine virus A (GVA) coat protein (CP) sequences were introduced into grapevine and the model plant Nicotiana benthamiana. The engineered constructs were tested for their ability to engender resistance in N. benthamiana. A number of GVA-CP-transformed N. benthamiana lines have become resistant to GVA. Preliminary biological and molecular analyses strongly suggest that in most cases the obtained resistance is RNA-mediated. Several lines of transgenic grapevine were obtained.

KW - Coat protein

KW - Grapevine

KW - Transgenic plants

KW - Virus resistance

KW - Vitivirus

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VL - 28

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EP - 86

JO - Phytoparasitica

JF - Phytoparasitica

IS - 1

ER -

Transgenic Nicotiana benthamiana and grapevine plants transformed with grapevine virus A (GVA) sequences

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Keywords

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