Translesion DNA synthesis across non-DNA segments in cultured human cells

Sheera Adar, Zvi Livneh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

DNA lesions that have escaped DNA repair are tolerated via translesion DNA synthesis (TLS), carried out by specialized error-prone DNA polymerases. To evaluate the robustness of the TLS system in human cells, we examined its ability to cope with foreign non-DNA stretches of 3 or 12 methylene residues, using a gap-lesion plasmid assay system. We found that both the trimethylene and dodecamethylene inserts were bypassed with significant efficiencies in human cells, using both misinsertion and misalignment mechanisms. TLS across these non-DNA segments was aphidicolin-sensitive, and did not require polη. In vitro primer extension assays showed that purified polη, polκ and polι were each capable of inserting each of the four nucleotides opposite the trimethylene chain, but only polη and polκ could fully bypass it. Polη and polι, but not polκ, could also insert each of the four nucleotides opposite the dodecamethylene chain, but all three polymerases were severely blocked by this lesion. The ability of TLS polymerases to insert nucleotides opposite a hydrocarbon chain, despite the lack of any similarity to DNA, suggests that they may act via a mode of transient and local template-independent polymerase activity, and highlights the robustness of the TLS system in human cells.

Original languageAmerican English
Pages (from-to)479-490
Number of pages12
JournalDNA Repair
Volume5
Issue number4
DOIs
StatePublished - 8 Apr 2006

Bibliographical note

Funding Information:
We thank M. Oren (Weizmann Institute, Rehovot, Israel) for the H1299 cell line, and A. Lehmann (Falmer, Brighton, UK) for the MRC5 and XP30RO cell lines. We thank Dr. Miriam Eisenstein (Department of Chemical Services, Weizmann Institute of Science) for modeling DNA with hydrocarbon inserts. This work was supported by grants from the Flight Attendant Medical Institute, Florida, USA, the Israel Science Foundation (no. 564/04), and by a grant from the M.D. Moross Institute for Cancer Research at the Weizmann Institute of Science.

Keywords

  • Carcinogenesis
  • DNA polymerase
  • Error-prone repair
  • Mutagenesis
  • TLR
  • TLS
  • Translesion replication

Fingerprint

Dive into the research topics of 'Translesion DNA synthesis across non-DNA segments in cultured human cells'. Together they form a unique fingerprint.

Cite this