Translocation of the Drosophila transient receptor potential-like (TRPL) channel requires both the N- and C-terminal regions together with sustained Ca2+ entry

David Richter, Ben Katz, Tina Oberacker, Vered Tzarfaty, Gregor Belusic, Baruch Minke, Armin Huber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

In Drosophila photoreceptors the transient receptor potential- like (TRPL), but not theTRPchannels undergo light-dependent translocation between the rhabdomere and cell body. Here we studied which of theTRPLchannel segments are essential for translocation and why the TRP channels are required for inducing TRPL translocation. We generated transgenic flies expressing chimeric TRP and TRPL proteins that formed functional light-activated channels. Translocation was induced only in chimera containing both the N- and C-terminal segments of TRPL. Using an inactive trp mutation and overexpressing the Na+/ Ca 2+ exchanger revealed that the essential function of the TRP channels in TRPL translocation is to enhance Ca2+-influx. These results indicate that motifs present at both the N and C termini as well as sustained Ca2+ entry are required for proper channel translocation.

Original languageEnglish
Pages (from-to)34234-34243
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number39
DOIs
StatePublished - 30 Oct 2011

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