TY - JOUR
T1 - Triacylglycerol synthesis in cultured rat hepatocytes
T2 - The rate‐limiting role of diacylglycerol acyltransferase
AU - MAYOREK, Nina
AU - GRINSTEIN, Irene
AU - BAR‐TANA, Jacob
PY - 1989/6
Y1 - 1989/6
N2 - The limiting role of diacylglycerol acyltransferase with respect to triacylglycerol synthesis in cultured rat hepatocytes was evaluated by following the inhibition of the overall synthetic flux by 2‐bromooctanoate acting as an inhibitor of the diacylglycerol acyltransferase step. The flux‐control coefficient of diacylglycerol acyltransferase in intact cultured hepatocytes amounted to 0.76 in the presence of saturating glycerol and either palmitate or oleate as the fatty acyl substrates. The flux‐control coefficient of diacylglycerol acyltransferase in lysolecithin‐permeabilized cultured hepatocytes amounted to 0.80 and 0.99 in the presence of saturating glycerol 3‐phosphate and either palmitate or oleate as the fatty acyl substrate, respectively. Hence, triacylglycerol synthesis in liver cells under the experimental conditions employed is rate‐limited by the diacylglycerol acyltransferase.
AB - The limiting role of diacylglycerol acyltransferase with respect to triacylglycerol synthesis in cultured rat hepatocytes was evaluated by following the inhibition of the overall synthetic flux by 2‐bromooctanoate acting as an inhibitor of the diacylglycerol acyltransferase step. The flux‐control coefficient of diacylglycerol acyltransferase in intact cultured hepatocytes amounted to 0.76 in the presence of saturating glycerol and either palmitate or oleate as the fatty acyl substrates. The flux‐control coefficient of diacylglycerol acyltransferase in lysolecithin‐permeabilized cultured hepatocytes amounted to 0.80 and 0.99 in the presence of saturating glycerol 3‐phosphate and either palmitate or oleate as the fatty acyl substrate, respectively. Hence, triacylglycerol synthesis in liver cells under the experimental conditions employed is rate‐limited by the diacylglycerol acyltransferase.
UR - http://www.scopus.com/inward/record.url?scp=0024351939&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1989.tb14844.x
DO - 10.1111/j.1432-1033.1989.tb14844.x
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C2 - 2737209
AN - SCOPUS:0024351939
SN - 0014-2956
VL - 182
SP - 395
EP - 400
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -