Trichoderma biocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry

Stanley Freeman*, Dror Minz, Inna Kolesnik, Olga Barbul, Aida Zveibil, Marcel Maymon, Yehuda Nitzani, Benny Kirshner, Dalia Rav-David, Alon Bilu, Arnon Dag, Sharoni Shafir, Yigal Elad

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

Trichoderma isolates are known for their ability to control plant pathogens. It has been shown that various isolates of Trichoderma, including T. harzianum isolate T-39 from the commercial biological control product TRICHODEX, were effective in controlling anthracnose (Colletotrichum acutatum) and grey mould (Botrytis cinerea) in strawberry, under controlled and greenhouse conditions. Three selected Trichoderma strains, namely T-39, T-161 and T-166, were evaluated in large-scale experiments using different timing application and dosage rates for reduction of strawberry anthracnose and grey mould. All possible combinations of single, double or triple mixtures of Trichoderma strains, applied at 0.4% and 0.8% concentrations, and at 7 or 10 day intervals, resulted in reduction of anthracnose severity; the higher concentration (0.8%) was superior in control whether used with single isolates or as a result of combined application of two isolates, each at 0.4%. Only a few treatments resulted in significant control of grey mould. Isolates T-39 applied at 0.4% at 2 day intervals, T-166 at 0.4%, or T-161 combined with T-39 at 0.4% were as effective as the chemical fungicide fenhexamide. The survival dynamics of populations of the Trichoderma isolates (T-39, T-105, T-161 and T-166) applied separately was determined by dilution plating and isolates in the mixtures calculated according to the polymerase chain reaction (PCR) using repeat motif primers. The biocontrol isolates were identified to the respective species T. harzianum (T-39), T. hamatum (T-105), T. atroviride (T-161) and T. longibrachiatum (T-166), according to internal transcribed spacer sequence analysis.

Original languageAmerican English
Pages (from-to)361-370
Number of pages10
JournalEuropean Journal of Plant Pathology
Volume110
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

Bibliographical note

Funding Information:
Contribution from the Agricultural Research Organization, Institute of Plant Protection, Bet Dagan, Israel, No. 500/03. The research was supported by the EU in the frame of FAIR project number CT 98-4140-IPM in strawberry.

Keywords

  • Anthracnose
  • Arbitrarily primed polymerase chain reaction (ap-PCR)
  • Fragaria x ananassa
  • Grey mould
  • Internal transcribed spacer (ITS) region
  • Ribosomal DNA

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