Tumor necrosis factors in a murine model of allergic peritonitis: Effects on eosinophil accumulation and inflammatory mediators' release

Vladislav Temkin, Dalia Pickholtz, Francesca Levi-Schaffer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

In allergic disorders, the role of tumor necrosis factors (TNF) is not well established. We investigated the role of TNF in allergic peritonitis induced by ovalbumin (OVA) challenge in double TNF (TNF-α -/-/lymphotoxin-α-/-) knock out (TNF-KO) mice. In the peritoneal lavage of TNF-KO mice, mast cell number and histamine level (radioenzymatic assay) were similar to that in wild type (WT) mice. TNF-α (ELISA) and histamine were increased 1 h after challenge in WT mice. However, three days later eosinophil number and eosinophil peroxidase (EPO) levels (colorimetric-enzymatic assay) were found to be lower in TNF-KO mice. A second challenge three days after the first, increased EPO, histamine and IL-6 (ELISA) but did not alter eosinophil and mast cell numbers in both types of mice. On the other hand histamine and IL-6 were higher, while EPO was lower in TNF-KO mice. In conclusion, our findings show that TNF is involved in eosinophil accumulation and inflammatory mediators' release in a murine model of allergy.

Original languageAmerican English
Pages (from-to)74-80
Number of pages7
JournalCytokine
Volume24
Issue number3
DOIs
StatePublished - 7 Nov 2003

Bibliographical note

Funding Information:
This work was supported by a grant from the Aimwell Charitable Trust (London, UK). Francesca Levi-Schaffer is affiliated to the David R. Bloom Center for Pharmacy at The Hebrew University of Jerusalem. Our thanks to Ms. Madelyn Segev for her secretarial assistance.

Keywords

  • Allergic peritonitis
  • Eosinophils
  • Histamine
  • Mast cells
  • TNF

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