TY - JOUR
T1 - Turgor Changes in Morchella esculenta during Translocation and Sclerotial Formation
AU - Amir, Rachel
AU - Steudle, Ernst
AU - Levanon, Dan
AU - Hadar, Yitzhak
AU - Chet, Ilan
PY - 1995/6
Y1 - 1995/6
N2 - Amir, R., Steudle, E., Levanon, D., Hadar, Y., and Chet, I. 1995. Turgor changes in Morchella esculenta during translocation and sclerotial formation. Experimental Mycology 19, 129-136. Turgor pressure was measured during six stages of growth and pseudosclerotial formation in Morchella esculenta indirectly (by thermocouple psychrometer) and directly (by cell pressure probe). The fungus was grown on a split plate, enabling separation between mycelium growing on defined medium (water potential -0.5 MPa) and sclerotia which formed on glucose noble agar (water potential -2.1 MPa). Under these conditions, nutrients were translocated from the mycelium to the developing sclerotia. Direct turgor potential measurements showed that the gradient between the mycelium and the sclerotia increases during sclerotial development (reaching a maximum of 0.53 MPa), thereby suggesting that translocation is a turgor-driven mass flow. During sclerotial development, the turgor potential in the peripheral tips of the sclerotial hyphae must be high enough to bring about the growth of the numerous hyphae, which comprise the sclerotium, and simultaneously low enough in the primary hyphae, which carry the stream of nutrients, to attract translocation from the mycelium. Since sclerotial hyphae are too small for direct measurement by cell pressure probe, a psychrometer was used, revealing high turgor in the sclerotial tissue (1.2 MPa) during selerotial development. Direct measurement in the primary hyphae at this time gave a value of 0.7 MPa. Taken together, these measurements indicate the presence of a turgor gradient inside the sclerotial tissue, from the primary hyphae to the peripheral cells. The present study is the first to make use of a cell pressure probe to measure turgor gradients in a fungus during translocation followed by sclerotial morphogenesis.
AB - Amir, R., Steudle, E., Levanon, D., Hadar, Y., and Chet, I. 1995. Turgor changes in Morchella esculenta during translocation and sclerotial formation. Experimental Mycology 19, 129-136. Turgor pressure was measured during six stages of growth and pseudosclerotial formation in Morchella esculenta indirectly (by thermocouple psychrometer) and directly (by cell pressure probe). The fungus was grown on a split plate, enabling separation between mycelium growing on defined medium (water potential -0.5 MPa) and sclerotia which formed on glucose noble agar (water potential -2.1 MPa). Under these conditions, nutrients were translocated from the mycelium to the developing sclerotia. Direct turgor potential measurements showed that the gradient between the mycelium and the sclerotia increases during sclerotial development (reaching a maximum of 0.53 MPa), thereby suggesting that translocation is a turgor-driven mass flow. During sclerotial development, the turgor potential in the peripheral tips of the sclerotial hyphae must be high enough to bring about the growth of the numerous hyphae, which comprise the sclerotium, and simultaneously low enough in the primary hyphae, which carry the stream of nutrients, to attract translocation from the mycelium. Since sclerotial hyphae are too small for direct measurement by cell pressure probe, a psychrometer was used, revealing high turgor in the sclerotial tissue (1.2 MPa) during selerotial development. Direct measurement in the primary hyphae at this time gave a value of 0.7 MPa. Taken together, these measurements indicate the presence of a turgor gradient inside the sclerotial tissue, from the primary hyphae to the peripheral cells. The present study is the first to make use of a cell pressure probe to measure turgor gradients in a fungus during translocation followed by sclerotial morphogenesis.
KW - cell pressure probe
KW - Morchella
KW - sclerotia
KW - translocation
KW - turgor
KW - water/osmotic potential
UR - http://www.scopus.com/inward/record.url?scp=0029062853&partnerID=8YFLogxK
U2 - 10.1006/emyc.1995.1015
DO - 10.1006/emyc.1995.1015
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AN - SCOPUS:0029062853
SN - 0147-5975
VL - 19
SP - 129
EP - 136
JO - Experimental Mycology
JF - Experimental Mycology
IS - 2
ER -