Two cleavage products of the Drosophila accessory gland protein ovulin can independently induce ovulation

Yael Heifetz, Laura N. Vandenberg, Heather I. Cohn, Mariana F. Wolfner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

Proteins and peptides in Drosophila melanogaster seminal fluid induce mated females to Increase their rates of egg deposition. One seminal-fluid protein, ovulin (Acp26Aa), stimulates an early step in the egg-laying process, the release of oocytes by the ovary. Ovulin, upon transfer to females, is cleaved sequentially within the mated female's reproductive tract. Here, we show that systemic ectopic expression of ovulin is sufficient to stimulate ovulation in unmated females. By using this assay to assess the functionality of ovulin's cleavage products, we find that two of the four cleavage products of ovulin can stimulate ovulation independently. Thus, ovulin's cleavage in mated females is not destructive and instead may liberate additional functional products with potential to modulate ovulation independently.

Original languageAmerican English
Pages (from-to)743-748
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number3
DOIs
StatePublished - 18 Jan 2005

Keywords

  • Mating
  • Processing
  • Prohormone
  • Seminal proteins

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