Two-dimensional mesoporous nitrogen-rich carbon nanosheets loaded with CeO2 nanoclusters as nanozymes for the electrochemical detection of superoxide anions in HepG2 cells

Zhenxing Wang, Hongli Zhao, Kaicha Chen, Fangfang Zhou, Shlomo Magdassi*, Minbo Lan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Two-dimensional (2D) porous carbon-based composite nanosheets loaded with metal oxide nanoclusters are expected to be promising electrocatalysts for high-performance electrochemical sensors. However, for this complicated composite material, strict reaction conditions and complex synthesis steps limit its general application in electrochemical detection. Here we present a facile method to fabricate 2D mesoporous nitrogen-rich carbon nanosheets loaded with CeO2 nanoclusters (2D-mNC@CeO2), for fabricating superoxide anions (O2•−) electrochemical sensor. The method is based on block copolymers self-assembly and the affinity of polydopamine to metal ions to obtain organic-inorganic hybrid, which can be directly converted into 2D-mNC@CeO2 through carbonization strategy without structural deterioration. Characterizations demonstrate that the 2D-mNC@CeO2 owned the 2D N-doped carbon structure with an interlinked hierarchical mesoporous and the uniformly dispersed CeO2 nanoclusters on the surface. Benefitted from the unique structure, the 2D-mNC@CeO2 shortens electron transfer distance, enhances mass transfer efficiency, exposes numerous active sites, and obtain a high Ce3+/Ce4+ ratio for improving electrocatalytic performance. The 2D-mNC@CeO2/SPCEs sensors for O2•− detection has a detection limit of 0.179 μM (S/N = 3) and sensitivity of 401.4 μA cm-2 mM-1. The sensors can be applied to capture electrochemical signals of O2•− released from HepG2 cells, demonstrating the application potential of the sensors to monitor O2•− in biological fields.

Original languageAmerican English
Article number114229
JournalBiosensors and Bioelectronics
Volume209
DOIs
StatePublished - 1 Aug 2022

Bibliographical note

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

  • Metal oxides nanoclusters
  • Nitrogen-rich carbon
  • Pore structure
  • Superoxide anions
  • Two-dimensional

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