Abstract
Multi-core fiber-bundle endoscopes provide a minimally-invasive solution for deep tissue imaging and opto-genetic stimulation, at depths beyond the reach of conventional microscopes. Recently, wavefront-shaping has enabled lensless bundle-based micro-endoscopy by correcting the wavefront distortions induced by core-to-core inhomogeneities. However, current wavefront-shaping solutions require access to the fiber distal end for determining the bend-sensitive wavefront-correction. Here, we show that it is possible to determine the wavefront correction in-situ, without any distal access. Exploiting the nonlinearity of two-photon excited fluorescence, we adaptively determine the wavefront correction in-situ using only proximal detection of epi-detected fluorescence. We experimentally demonstrate diffraction-limited, three-dimensional, two-photon lensless microendoscopy with commercially-available orderedand disordered multi-core fiber bundles.
| Original language | English |
|---|---|
| Pages (from-to) | 28807-28817 |
| Number of pages | 11 |
| Journal | Optics Express |
| Volume | 26 |
| Issue number | 22 |
| DOIs | |
| State | Published - 29 Oct 2018 |
Bibliographical note
Publisher Copyright:© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.