Two-photon lensless micro-endoscopy with in-situ wavefront correction

Uri Weiss, Ori Katz

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Multi-core fiber-bundle endoscopes provide a minimally-invasive solution for deep tissue imaging and opto-genetic stimulation, at depths beyond the reach of conventional microscopes. Recently, wavefront-shaping has enabled lensless bundle-based micro-endoscopy by correcting the wavefront distortions induced by core-to-core inhomogeneities. However, current wavefront-shaping solutions require access to the fiber distal end for determining the bend-sensitive wavefront-correction. Here, we show that it is possible to determine the wavefront correction in-situ, without any distal access. Exploiting the nonlinearity of two-photon excited fluorescence, we adaptively determine the wavefront correction in-situ using only proximal detection of epi-detected fluorescence. We experimentally demonstrate diffraction-limited, three-dimensional, two-photon lensless microendoscopy with commercially-available orderedand disordered multi-core fiber bundles.

Original languageAmerican English
Pages (from-to)28807-28817
Number of pages11
JournalOptics Express
Issue number22
StatePublished - 29 Oct 2018

Bibliographical note

Publisher Copyright:
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.


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