Two regulatory elements for immunoglobulin κ light chain gene expression

Y. Bergman, D. Rice, R. Grosschedl, D. Baltimore

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

By using internal deletions within a rear-ranged immunoglobulin κ light chain gene, the presence of an intron regulatory sequence (enhancer) has been confirmed. Its presence is required for high-level transcription from a plasmid after transfection into myeloma cells. Transfection efficiency was monitored by the activity of a deleted H4 histone gene included in the plasmid. The intron element could be moved upstream of the gene in both orientations, fulfilling the definition of an enhancer. By using 5' deletions, a second regulatory element was located upstream of the 'TATA' box, between positions -69 and -104. These two elements both are required for efficient κ chain gene expression.

Original languageAmerican English
Pages (from-to)7041-7045
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number22 I
DOIs
StatePublished - 1984
Externally publishedYes

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