Typing of halophilic Archaea and characterization of their cell surface carbohydrates by use of lectins

Nechama Gilboa-Garber*, Hana Mymon, Aharon Oren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Lectins are important tools for cell typing and for the study of cell surface components. They have been widely used for the analysis of carbohydrates on the surface of many eukaryotic and prokaryotic cells, but they have not yet been exploited in the study of the halophilic Archaea (family Halobacteriaceae), because of the high salinity required for the structural integrity of these microorganisms. We have defined the salt concentration threshold high enough for survival of the Archaea, but sufficiently low for lectins to bind to them. Under these conditions we studied the interactions of a series of lectins, exhibiting different sugar specificities, with diverse halophilic Archaea. Concanavalin A was the most reactive by virtue of its glucose (and mannose) binding. The other lectins varied in their interactions. The results indicate that lectins might be useful probes for both archaeal typing and analysis of their cell surface carbohydrates.

Original languageAmerican English
Pages (from-to)91-97
Number of pages7
JournalFEMS Microbiology Letters
Issue number1
StatePublished - 1 Jun 1998

Bibliographical note

Funding Information:
The work was supported by the Bar-Ilan University Research Foundation. We thank Avrille Goldreich, Mrs. Sharon Victor and Mrs. Ella Gindi for the skillful typing of the manuscript and the graphic presentation.


  • Archaeal glycoprotein
  • Archaeal typing
  • Lectin
  • Sugar detection


Dive into the research topics of 'Typing of halophilic Archaea and characterization of their cell surface carbohydrates by use of lectins'. Together they form a unique fingerprint.

Cite this