Tyrphostin-induced differentiation of mouse erythroleukemia cells

Mordechai Anafi; Aviv Gazit; Chaim Gilon; Yinon Ben Neriah; Alexander Levitzki

doi:10.1016/0014-5793(93)80884-W

Tyrphostin-induced differentiation of mouse erythroleukemia cells

Mordechai Anafi^*, Aviv Gazit, Chaim Gilon, Yinon Ben Neriah, Alexander Levitzki

^*Corresponding author for this work

Department of Immunology and Cancer Research

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Inhibitors of protein-tyrosine kinases (TPKs) from the tyrphostins family induce terminal erythroid differentiation of mouse erythroleukemia (MEL) cells. The most potent tyrphostin was found to be AG-568 which was therefore investigated in more detail. Just prior to differentiation the inhibition of tyrosine phosphorylation of a pp⁹⁷ protein band was noted. We also found that AG-568 treatment induces the appearance of a putative differentiation factor which could induce tyrphostin-independent differentiation in untreated cells. Our study suggests that the inhibition of tyrosine phosphorylation by AG-568 leads to the production of differentiating factor(s) which induce the MEL cells to differentiate.

Original language	English
Pages (from-to)	260-264
Number of pages	5
Journal	FEBS Letters
Volume	330
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80884-W
State	Published - 20 Sep 1993

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abstract = "Inhibitors of protein-tyrosine kinases (TPKs) from the tyrphostins family induce terminal erythroid differentiation of mouse erythroleukemia (MEL) cells. The most potent tyrphostin was found to be AG-568 which was therefore investigated in more detail. Just prior to differentiation the inhibition of tyrosine phosphorylation of a pp97 protein band was noted. We also found that AG-568 treatment induces the appearance of a putative differentiation factor which could induce tyrphostin-independent differentiation in untreated cells. Our study suggests that the inhibition of tyrosine phosphorylation by AG-568 leads to the production of differentiating factor(s) which induce the MEL cells to differentiate.",

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AU - Gilon, Chaim

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AU - Levitzki, Alexander

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AB - Inhibitors of protein-tyrosine kinases (TPKs) from the tyrphostins family induce terminal erythroid differentiation of mouse erythroleukemia (MEL) cells. The most potent tyrphostin was found to be AG-568 which was therefore investigated in more detail. Just prior to differentiation the inhibition of tyrosine phosphorylation of a pp97 protein band was noted. We also found that AG-568 treatment induces the appearance of a putative differentiation factor which could induce tyrphostin-independent differentiation in untreated cells. Our study suggests that the inhibition of tyrosine phosphorylation by AG-568 leads to the production of differentiating factor(s) which induce the MEL cells to differentiate.

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Tyrphostin-induced differentiation of mouse erythroleukemia cells

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