Tyrphostin-induced differentiation of mouse erythroleukemia cells

Mordechai Anafi*, Aviv Gazit, Chaim Gilon, Yinon Ben Neriah, Alexander Levitzki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Inhibitors of protein-tyrosine kinases (TPKs) from the tyrphostins family induce terminal erythroid differentiation of mouse erythroleukemia (MEL) cells. The most potent tyrphostin was found to be AG-568 which was therefore investigated in more detail. Just prior to differentiation the inhibition of tyrosine phosphorylation of a pp97 protein band was noted. We also found that AG-568 treatment induces the appearance of a putative differentiation factor which could induce tyrphostin-independent differentiation in untreated cells. Our study suggests that the inhibition of tyrosine phosphorylation by AG-568 leads to the production of differentiating factor(s) which induce the MEL cells to differentiate.

Original languageAmerican English
Pages (from-to)260-264
Number of pages5
JournalFEBS Letters
Issue number3
StatePublished - 20 Sep 1993


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