Ultradense synthetic gene brushes on a chip

Amnon Buxboim, Shirley S. Daube, Roy Bar-Ziv*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Dense brushes of linear DNA polymers are assembled on a blochlp with ∼30 nm between anchorage points, amounting to a few mega-base-pairs/ μm3. In bulk solution, a barrier Incurs to conjugate more than two end-functionalized DNAs. However, such doublets bind the surface with almost equal efficiency to singlets, suggesting that extended brush buildup reduces the barrier. On-chip transcription reveals that doublets are roughly 2-fold Inefficient compared to singlets, a manifestation of the Interaction of the enzymatic machinery with the dense brush. Synthetic gene brushes made of DNA conjugates provide simple means to regulate expression on a chip.

Original languageAmerican English
Pages (from-to)909-913
Number of pages5
JournalNano Letters
Volume9
Issue number2
DOIs
StatePublished - 11 Feb 2009
Externally publishedYes

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