Ultramicrodetermination of Amino Acids and Histidyl-Peptides By Fluorescence Measurements of Spots on Paper

R. Reisfeld; S. Levi

doi:10.1080/00032717708079392

Ultramicrodetermination of Amino Acids and Histidyl-Peptides By Fluorescence Measurements of Spots on Paper

R. Reisfeld, S. Levi

Institute of Chemistry

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Abstract

A method was developed which ensures reproducibility of fluorescence of OPT histidine and histidyl-peptides, and other amino acid-ninhydrin-phenylacetaldehyde complexes absorbed on filter paper. A linear dependence of fluorescence was found in the range 2–400 ng for histidine and histidyl-peptides with the OPT reagent, and in the range 7–250 ng for glycine, phenylalanine, histidine and methionine, with the ninhydrin-phenylacetaldehyde reagent.

Original language	English
Pages (from-to)	483-492
Number of pages	10
Journal	Analytical Letters
Volume	10
Issue number	6
DOIs	https://doi.org/10.1080/00032717708079392
State	Published - 1 Jan 1977

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10.1080/00032717708079392

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abstract = "A method was developed which ensures reproducibility of fluorescence of OPT histidine and histidyl-peptides, and other amino acid-ninhydrin-phenylacetaldehyde complexes absorbed on filter paper. A linear dependence of fluorescence was found in the range 2–400 ng for histidine and histidyl-peptides with the OPT reagent, and in the range 7–250 ng for glycine, phenylalanine, histidine and methionine, with the ninhydrin-phenylacetaldehyde reagent.",

author = "R. Reisfeld and S. Levi",

year = "1977",

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N2 - A method was developed which ensures reproducibility of fluorescence of OPT histidine and histidyl-peptides, and other amino acid-ninhydrin-phenylacetaldehyde complexes absorbed on filter paper. A linear dependence of fluorescence was found in the range 2–400 ng for histidine and histidyl-peptides with the OPT reagent, and in the range 7–250 ng for glycine, phenylalanine, histidine and methionine, with the ninhydrin-phenylacetaldehyde reagent.

AB - A method was developed which ensures reproducibility of fluorescence of OPT histidine and histidyl-peptides, and other amino acid-ninhydrin-phenylacetaldehyde complexes absorbed on filter paper. A linear dependence of fluorescence was found in the range 2–400 ng for histidine and histidyl-peptides with the OPT reagent, and in the range 7–250 ng for glycine, phenylalanine, histidine and methionine, with the ninhydrin-phenylacetaldehyde reagent.

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Ultramicrodetermination of Amino Acids and Histidyl-Peptides By Fluorescence Measurements of Spots on Paper

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