Ultramicrodetermination of cholesterol, testosterone and LSD by fluorescence measurements of TLC spots

S. Levi*, R. Reisfeld

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Fluorescent derivatives of many organic materials adsorbed on t.l.c. plates are obtained by reaction with ammonia liberated by heating ammonium hydrogencarbonate. Spots whose fluorescence can be measured quantitatively with high sensitivity over wide ranges of concentrations can be obtained with testosterone (1-250 ng), LSD (2-150 ng) and cholesterol (4-150 ng).

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalAnalytica Chimica Acta
Volume97
Issue number2
DOIs
StatePublished - 1 Apr 1978

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