TY - JOUR
T1 - "Ultramicroinjection" of macromolecules or small particles into animal cells
T2 - A new technique based on virus-induced cell fusion
AU - Loyter, Abraham
AU - Zakai, Nehama
AU - Kulka, Richard G.
PY - 1975/8/1
Y1 - 1975/8/1
N2 - A new method is described for the introduction of macromolecules and small particles into animal cells. The first step in this procedure is the trapping of particles in ghosts of human erythrocytes. This is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. The second step is the Sendai virus-induced fusion of the ghosts containing the particles with cells. By this method, ferritin and latex spheres (diameter 0.1 µm) have been "injected" into cells.
AB - A new method is described for the introduction of macromolecules and small particles into animal cells. The first step in this procedure is the trapping of particles in ghosts of human erythrocytes. This is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. The second step is the Sendai virus-induced fusion of the ghosts containing the particles with cells. By this method, ferritin and latex spheres (diameter 0.1 µm) have been "injected" into cells.
UR - http://www.scopus.com/inward/record.url?scp=0016537592&partnerID=8YFLogxK
U2 - 10.1083/jcb.66.2.292
DO - 10.1083/jcb.66.2.292
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C2 - 167032
AN - SCOPUS:0016537592
SN - 0021-9525
VL - 66
SP - 292
EP - 304
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -
