Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets: Preliminary indications for the presence of contractile proteins

J. Sela*, I. A. Bab, A. Muhlbad

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca2-, Mg2+-, and K+ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg2+-, and Ca2+-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K+ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalMetabolic Bone Disease and Related Research
Volume1
Issue number3
DOIs
StatePublished - 1978

Keywords

  • Alkaline phosphatase
  • Contractile proteins
  • Healing socket
  • Matrix vesicles

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