TY - JOUR
T1 - Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets
T2 - Preliminary indications for the presence of contractile proteins
AU - Sela, J.
AU - Bab, I. A.
AU - Muhlbad, A.
PY - 1978
Y1 - 1978
N2 - Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca2-, Mg2+-, and K+ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg2+-, and Ca2+-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K+ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.
AB - Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca2-, Mg2+-, and K+ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg2+-, and Ca2+-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K+ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.
KW - Alkaline phosphatase
KW - Contractile proteins
KW - Healing socket
KW - Matrix vesicles
UR - http://www.scopus.com/inward/record.url?scp=0006667960&partnerID=8YFLogxK
U2 - 10.1016/0221-8747(78)90001-2
DO - 10.1016/0221-8747(78)90001-2
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AN - SCOPUS:0006667960
SN - 0221-8747
VL - 1
SP - 185
EP - 191
JO - Metabolic Bone Disease and Related Research
JF - Metabolic Bone Disease and Related Research
IS - 3
ER -