Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets: Preliminary indications for the presence of contractile proteins

J. Sela; I. A. Bab; A. Muhlbad

doi:10.1016/0221-8747(78)90001-2

Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets: Preliminary indications for the presence of contractile proteins

J. Sela^*
, I. A. Bab
, A. Muhlbad

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca^2-, Mg²⁺-, and K⁺ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg²⁺-, and Ca²⁺-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K⁺ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.

Original language	English
Pages (from-to)	185-191
Number of pages	7
Journal	Metabolic Bone Disease and Related Research
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/0221-8747(78)90001-2
State	Published - 1978

Keywords

Alkaline phosphatase
Contractile proteins
Healing socket
Matrix vesicles

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10.1016/0221-8747(78)90001-2

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title = "Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets: Preliminary indications for the presence of contractile proteins",

abstract = "Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca2-, Mg2+-, and K+ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg2+-, and Ca2+-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K+ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.",

keywords = "Alkaline phosphatase, Contractile proteins, Healing socket, Matrix vesicles",

author = "J. Sela and Bab, \{I. A.\} and A. Muhlbad",

year = "1978",

doi = "10.1016/0221-8747(78)90001-2",

language = "אנגלית",

volume = "1",

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journal = "Metabolic Bone Disease and Related Research",

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T1 - Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets

T2 - Preliminary indications for the presence of contractile proteins

AU - Sela, J.

AU - Bab, I. A.

AU - Muhlbad, A.

PY - 1978

Y1 - 1978

N2 - Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca2-, Mg2+-, and K+ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg2+-, and Ca2+-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K+ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.

AB - Extracellular matrix vesicles were identified and isolated from healing bone sockets. The diameters of these trilaminar membrane-invested organelles was 0.05 to OAS μm. Ca2-, Mg2+-, and K+ (EDTA)-mediated ATPases; alkaline and acid phosphatase; and pyrophosphatase activities of isolated matrix vesicles were compared with the membrane and cellular fractions. The vesicle fraction showed high alkaline phosphatase activity, which correlated with Mg2+-, and Ca2+-mediated ATPases. Pyrophosphatase activity was highest in the vesicle fraction as well. The presence of K+ (EDTA)-mediated ATPase in all the fractions indicated the occurrence of myosin. These findings were supported by electrophoretic patterns which revealed the presence of another contractile protein-actin.

KW - Alkaline phosphatase

KW - Contractile proteins

KW - Healing socket

KW - Matrix vesicles

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SN - 0221-8747

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JO - Metabolic Bone Disease and Related Research

JF - Metabolic Bone Disease and Related Research

IS - 3

ER -

Ultrastructural and biochemical characterization of extracellular matrix vesicles in healing alveolar bone sockets: Preliminary indications for the presence of contractile proteins

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Keywords

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