TY - GEN
T1 - UNIQUE REGULATORY PROPERTIES OF THE UDP-GLUCOSE
T2 - beta -1,4-GLUCAN SYNTHETASE OF ACETOBACTER XYLINUM.
AU - Benziman, Moshe
AU - Aloni, Yehoshua
AU - Delmer, Deborah P.
PY - 1983
Y1 - 1983
N2 - Conditions have been found for an extremely efficient transfer of glucose from UDP-glucose to a cellulosic beta -1,4-glucan product, using enzyme preparations derived from cells of Acetobacter xylinum. Membrane fractions obtained by rupturing cells in the presence of 20% (w/v) polyethylene glycol-4000 (PEG-4000) exhibited UDP-glucose: beta -1,4-glucan synthetase activity 3- to 10-fold higher than those previously reported. Enzyme prepared in this fashion also shows a further marked activation by GTP. Enzyme prepared in the absence of PEG-4000 does not respond to GTP because it lacks a protein factor essential for GTP activation. Under optimal conditions in the presence of GTP, factor, and PEG-4000, initial rates of enzyme activity that are 200 times higher than those previously reported can be achieved. Such rates exceed 40% of the in vivo rate of cellulose synthesis from glucose.
AB - Conditions have been found for an extremely efficient transfer of glucose from UDP-glucose to a cellulosic beta -1,4-glucan product, using enzyme preparations derived from cells of Acetobacter xylinum. Membrane fractions obtained by rupturing cells in the presence of 20% (w/v) polyethylene glycol-4000 (PEG-4000) exhibited UDP-glucose: beta -1,4-glucan synthetase activity 3- to 10-fold higher than those previously reported. Enzyme prepared in this fashion also shows a further marked activation by GTP. Enzyme prepared in the absence of PEG-4000 does not respond to GTP because it lacks a protein factor essential for GTP activation. Under optimal conditions in the presence of GTP, factor, and PEG-4000, initial rates of enzyme activity that are 200 times higher than those previously reported can be achieved. Such rates exceed 40% of the in vivo rate of cellulose synthesis from glucose.
UR - http://www.scopus.com/inward/record.url?scp=0020991125&partnerID=8YFLogxK
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AN - SCOPUS:0020991125
SN - 0471881325
T3 - Applied Polymer Symposia
SP - 131
EP - 143
BT - Applied Polymer Symposia
PB - John Wiley & Sons
ER -