Unraveling chemokine and chemokine receptor expression patterns using genetically engineered mice

Simon Yona, Ki Wook Kim, Rebecca Haffner, Steffen Jung

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Over the past 25 years, genetically engineered mouse models have become an integral and invaluable research tool to develop our understanding of mammalian physiology and pathology. This unit describes methods for generating transgenic mice, focusing on reporter animals relevant to chemokine receptor and ligand expression. Speci fi cally, we describe the use of bacterial arti fi cial chromosome (BAC) engineering and embryonic stem cell manipulation to generate " knock in " and transgenic mice.

Original languageEnglish
Title of host publicationChemokines
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages129-144
Number of pages16
ISBN (Print)9781627034258
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1013
ISSN (Print)1064-3745

Keywords

  • Bacterial arti fi cial chromosome
  • Chemokines
  • Embryonic stem cells and mice

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