Use of fluorescent imaging to investigate pathological protease activity

G. Blum*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

38 Scopus citations

Abstract

Proteases play pivotal roles in the normal function of cells. In addition, the expression and activity of proteases are significantly upregulated in several pathologies, including cancer, arthritis and atherosclerosis, and hence they can be considered to be biological markers for these pathologies. The hydrolyzing activity of proteases has been used to generate a variety of fluorescent imaging reagents, the design and utility of which are reviewed here. The use of imaging reagents to visualize protease activity allows for improved detection of various pathologies as well as the ability to monitor the efficacy of therapies in vivo and provide molecular information regarding the nature of the pathology.

Original languageAmerican English
Pages (from-to)708-716
Number of pages9
JournalCurrent Opinion in Drug Discovery and Development
Volume11
Issue number5
StatePublished - Sep 2008
Externally publishedYes

Keywords

  • Activity-based probes
  • Biomarkers
  • Fluorescence
  • Optical imaging
  • Proteases
  • Substrate

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