Use of Nitrogen-15 Determinations of Purine Nitrogen Fraction of Digesta to Define Nitrogen Metabolism Traits in the Rumen

Yoav Aharoni*, Haim Tagari

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for 15N determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of 15N in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the 15N enrichment curves between diets based on either roasted or raw soybean meal.

Original languageEnglish
Pages (from-to)2540-2547
Number of pages8
JournalJournal of Dairy Science
Volume74
Issue number8
DOIs
StatePublished - 1991

Keywords

  • BS
  • buffer solution
  • dialyzer solution
  • DS
  • NA
  • NB
  • nitrogen-15
  • nucleic acids
  • nucleic bases
  • PN
  • purine nitrogen
  • purine nitrogen
  • raw soybean meal
  • roasted soybean meal
  • RSBM
  • rumen
  • SBM
  • SP
  • spectrophotometric procedure

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