Abstract
A method for direct purine N isolation and determination was modified from the method of Zinn and Owens for rapid determination of purines. By this method, N derived from purines in digesta was measured and collected for 15N determinations. Measurements of purine N in 33 samples of ruminal contents and duodenal digesta of sheep and goats were compared with purine determinations in the same samples. The results showed that essentially all the N in the purines that could be isolated by the Zinn and Owens procedure also could be detected by N isolation and determination. Determinations of 15N in the NH3 N, NAN, and purine N fractions in a continuous culture in vitro showed significant differences in the 15N enrichment curves between diets based on either roasted or raw soybean meal.
Original language | English |
---|---|
Pages (from-to) | 2540-2547 |
Number of pages | 8 |
Journal | Journal of Dairy Science |
Volume | 74 |
Issue number | 8 |
DOIs | |
State | Published - 1991 |
Keywords
- BS
- buffer solution
- dialyzer solution
- DS
- NA
- NB
- nitrogen-15
- nucleic acids
- nucleic bases
- PN
- purine nitrogen
- purine nitrogen
- raw soybean meal
- roasted soybean meal
- RSBM
- rumen
- SBM
- SP
- spectrophotometric procedure