TY - JOUR
T1 - Use of the RAPD procedure for the identification of Trichoderma strains
AU - Zimand, Gilly
AU - Valinsky, Lea
AU - Elad, Y.
AU - Chet, I.
AU - Manulis, Shulamit
PY - 1994
Y1 - 1994
N2 - Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T. harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.
AB - Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T. harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0028039484&partnerID=8YFLogxK
U2 - 10.1016/S0953-7562(09)80474-7
DO - 10.1016/S0953-7562(09)80474-7
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AN - SCOPUS:0028039484
SN - 0953-7562
VL - 98
SP - 531
EP - 534
JO - Mycological Research
JF - Mycological Research
IS - 5
ER -
