Use of the RAPD procedure for the identification of Trichoderma strains

Gilly Zimand; Lea Valinsky; Y. Elad; I. Chet; Shulamit Manulis

doi:10.1016/S0953-7562(09)80474-7

Use of the RAPD procedure for the identification of Trichoderma strains

Gilly Zimand
, Lea Valinsky
, Y. Elad^*
, I. Chet
, Shulamit Manulis

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T. harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.

Original language	English
Pages (from-to)	531-534
Number of pages	4
Journal	Mycological Research
Volume	98
Issue number	5
DOIs	https://doi.org/10.1016/S0953-7562(09)80474-7
State	Published - 1994

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10.1016/S0953-7562(09)80474-7

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title = "Use of the RAPD procedure for the identification of Trichoderma strains",

abstract = "Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T. harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.",

author = "Gilly Zimand and Lea Valinsky and Y. Elad and I. Chet and Shulamit Manulis",

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AU - Elad, Y.

AU - Chet, I.

AU - Manulis, Shulamit

PY - 1994

Y1 - 1994

N2 - Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T. harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.

AB - Using the RAPD (random amplified polymorphic DNA) procedure with arbitrary 10 oligonucleotide primers, we were able to distinguish among strains of Trichoderma. Ten of the strains which were identified as T. harzianum exhibited similar patterns. Among the strains of T. viride, only three pairs yielded similar patterns. None of the strains identified as T. hamatum showed any similarities. The isolate T-39, which is used commercially as a biocontrol agent against Botrytis cinerea, was distinguishable by this procedure. The use of this method for the precise identification of Trichoderma strains is discussed.

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JO - Mycological Research

JF - Mycological Research

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ER -

Use of the RAPD procedure for the identification of Trichoderma strains

Abstract

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