Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates

Jan Jehlička; Adam Culka; Lilly Mana; Aharon Oren

doi:10.1007/s00216-018-1098-3

Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates

Jan Jehlička^*, Adam Culka, Lilly Mana, Aharon Oren

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO₄·7H₂O, K₂SO₄, and (NH₄)Al(SO₄)₂·12H₂O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200–4000 cm⁻¹. This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.

Original language	American English
Pages (from-to)	4437-4443
Number of pages	7
Journal	Analytical and Bioanalytical Chemistry
Volume	410
Issue number	18
DOIs	https://doi.org/10.1007/s00216-018-1098-3
State	Published - 1 Jul 2018

Bibliographical note

Funding Information:
Acknowledgments This study was funded by the Czech Science Foundation Project 17-04270S. This research was also supported by Center for Geosphere Dynamics (UNCE/SCI/006). AO was supported by grant no. 2221/15 from the Israel Science Foundation. This study was further supported by the Erasmus+ inter-institutional agreement between the Charles University, Prague, and the Hebrew University of Jerusalem.

Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Carotenoids
Halophilic prokaryotes
Inclusions
Miniature Raman spectrometer
Salts

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10.1007/s00216-018-1098-3

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title = "Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates",

abstract = "Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4·7H2O, K2SO4, and (NH4)Al(SO4)2·12H2O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200–4000 cm−1. This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.",

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author = "Jan Jehli{\v c}ka and Adam Culka and Lilly Mana and Aharon Oren",

note = "Funding Information: Acknowledgments This study was funded by the Czech Science Foundation Project 17-04270S. This research was also supported by Center for Geosphere Dynamics (UNCE/SCI/006). AO was supported by grant no. 2221/15 from the Israel Science Foundation. This study was further supported by the Erasmus+ inter-institutional agreement between the Charles University, Prague, and the Hebrew University of Jerusalem. Publisher Copyright: {\textcopyright} 2018, Springer-Verlag GmbH Germany, part of Springer Nature.",

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AU - Oren, Aharon

N1 - Funding Information: Acknowledgments This study was funded by the Czech Science Foundation Project 17-04270S. This research was also supported by Center for Geosphere Dynamics (UNCE/SCI/006). AO was supported by grant no. 2221/15 from the Israel Science Foundation. This study was further supported by the Erasmus+ inter-institutional agreement between the Charles University, Prague, and the Hebrew University of Jerusalem. Publisher Copyright: © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

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N2 - Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4·7H2O, K2SO4, and (NH4)Al(SO4)2·12H2O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200–4000 cm−1. This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.

AB - Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4·7H2O, K2SO4, and (NH4)Al(SO4)2·12H2O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200–4000 cm−1. This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.

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Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates

Abstract

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Keywords

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