Uterine washings as a novel method for early detection of ovarian cancer: Trials and tribulations

Tiffany Y. Sia; Zvi Yaari; Ron Feiner; Evan Smith; Arnaud Da Cruz Paula; Pier Selenica; Sital Doddi; Dennis S. Chi; Nadeem R. Abu-Rustum; Douglas A. Levine; Britta Weigelt; Martin Fleisher; Lakshmi V. Ramanathan; Daniel A. Heller; Kara Long Roche

doi:10.1016/j.gore.2024.101330

Uterine washings as a novel method for early detection of ovarian cancer: Trials and tribulations

Tiffany Y. Sia, Zvi Yaari, Ron Feiner, Evan Smith, Arnaud Da Cruz Paula, Pier Selenica, Sital Doddi, Dennis S. Chi, Nadeem R. Abu-Rustum, Douglas A. Levine, Britta Weigelt, Martin Fleisher, Lakshmi V. Ramanathan, Daniel A. Heller, Kara Long Roche^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

Given the tubal origin of high-grade serous ovarian cancer (HGSC), we sought to investigate intrauterine lavage (IUL) as a novel method of biomarker detection. IUL and serum samples were collected from patients with HGSC or benign pathology. Although CA-125 and HE4 concentrations were significantly higher in IUL samples compared to serum, they were similar between IUL samples from patients with HGSC vs benign conditions. In contrast, CA-125 and HE4 serum concentrations differed between HGSC and benign pathology (P =.002 for both). IUL and tumor samples from patients with HGSC were subjected to targeted panel sequencing and droplet digital PCR (ddPCR). Tumor mutations were found in 75 % of matched IUL samples. Serum CA-125 and HE4 biomarker levels allowed for better differentiation of HGSC and benign pathology compared to IUL samples. We believe using IUL for early detection of HGSC requires optimization, and current strategies should focus on prevention until early detection strategies improve.

Original language	English
Article number	101330
Journal	Gynecologic Oncology Reports
Volume	51
DOIs	https://doi.org/10.1016/j.gore.2024.101330
State	Published - Feb 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Keywords

Early detection
Intrauterine lavage
Ovarian cancer

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10.1016/j.gore.2024.101330

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Sia, T. Y., Yaari, Z., Feiner, R., Smith, E., Da Cruz Paula, A., Selenica, P., Doddi, S., Chi, D. S., Abu-Rustum, N. R., Levine, D. A., Weigelt, B., Fleisher, M., Ramanathan, L. V., Heller, D. A., & Long Roche, K. (2024). Uterine washings as a novel method for early detection of ovarian cancer: Trials and tribulations. Gynecologic Oncology Reports, 51, Article 101330. https://doi.org/10.1016/j.gore.2024.101330

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title = "Uterine washings as a novel method for early detection of ovarian cancer: Trials and tribulations",

abstract = "Given the tubal origin of high-grade serous ovarian cancer (HGSC), we sought to investigate intrauterine lavage (IUL) as a novel method of biomarker detection. IUL and serum samples were collected from patients with HGSC or benign pathology. Although CA-125 and HE4 concentrations were significantly higher in IUL samples compared to serum, they were similar between IUL samples from patients with HGSC vs benign conditions. In contrast, CA-125 and HE4 serum concentrations differed between HGSC and benign pathology (P =.002 for both). IUL and tumor samples from patients with HGSC were subjected to targeted panel sequencing and droplet digital PCR (ddPCR). Tumor mutations were found in 75 % of matched IUL samples. Serum CA-125 and HE4 biomarker levels allowed for better differentiation of HGSC and benign pathology compared to IUL samples. We believe using IUL for early detection of HGSC requires optimization, and current strategies should focus on prevention until early detection strategies improve.",

keywords = "Early detection, Intrauterine lavage, Ovarian cancer",

author = "Sia, {Tiffany Y.} and Zvi Yaari and Ron Feiner and Evan Smith and {Da Cruz Paula}, Arnaud and Pier Selenica and Sital Doddi and Chi, {Dennis S.} and Abu-Rustum, {Nadeem R.} and Levine, {Douglas A.} and Britta Weigelt and Martin Fleisher and Ramanathan, {Lakshmi V.} and Heller, {Daniel A.} and {Long Roche}, Kara",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = feb,

doi = "10.1016/j.gore.2024.101330",

language = "אנגלית",

volume = "51",

journal = "Gynecologic Oncology Reports",

issn = "2211-338X",

publisher = "Elsevier BV",

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Sia, TY, Yaari, Z, Feiner, R, Smith, E, Da Cruz Paula, A, Selenica, P, Doddi, S, Chi, DS, Abu-Rustum, NR, Levine, DA, Weigelt, B, Fleisher, M, Ramanathan, LV, Heller, DA & Long Roche, K 2024, 'Uterine washings as a novel method for early detection of ovarian cancer: Trials and tribulations', Gynecologic Oncology Reports, vol. 51, 101330. https://doi.org/10.1016/j.gore.2024.101330

TY - JOUR

T1 - Uterine washings as a novel method for early detection of ovarian cancer

T2 - Trials and tribulations

AU - Sia, Tiffany Y.

AU - Yaari, Zvi

AU - Feiner, Ron

AU - Smith, Evan

AU - Da Cruz Paula, Arnaud

AU - Selenica, Pier

AU - Doddi, Sital

AU - Chi, Dennis S.

AU - Abu-Rustum, Nadeem R.

AU - Levine, Douglas A.

AU - Weigelt, Britta

AU - Fleisher, Martin

AU - Ramanathan, Lakshmi V.

AU - Heller, Daniel A.

AU - Long Roche, Kara

PY - 2024/2

Y1 - 2024/2

N2 - Given the tubal origin of high-grade serous ovarian cancer (HGSC), we sought to investigate intrauterine lavage (IUL) as a novel method of biomarker detection. IUL and serum samples were collected from patients with HGSC or benign pathology. Although CA-125 and HE4 concentrations were significantly higher in IUL samples compared to serum, they were similar between IUL samples from patients with HGSC vs benign conditions. In contrast, CA-125 and HE4 serum concentrations differed between HGSC and benign pathology (P =.002 for both). IUL and tumor samples from patients with HGSC were subjected to targeted panel sequencing and droplet digital PCR (ddPCR). Tumor mutations were found in 75 % of matched IUL samples. Serum CA-125 and HE4 biomarker levels allowed for better differentiation of HGSC and benign pathology compared to IUL samples. We believe using IUL for early detection of HGSC requires optimization, and current strategies should focus on prevention until early detection strategies improve.

AB - Given the tubal origin of high-grade serous ovarian cancer (HGSC), we sought to investigate intrauterine lavage (IUL) as a novel method of biomarker detection. IUL and serum samples were collected from patients with HGSC or benign pathology. Although CA-125 and HE4 concentrations were significantly higher in IUL samples compared to serum, they were similar between IUL samples from patients with HGSC vs benign conditions. In contrast, CA-125 and HE4 serum concentrations differed between HGSC and benign pathology (P =.002 for both). IUL and tumor samples from patients with HGSC were subjected to targeted panel sequencing and droplet digital PCR (ddPCR). Tumor mutations were found in 75 % of matched IUL samples. Serum CA-125 and HE4 biomarker levels allowed for better differentiation of HGSC and benign pathology compared to IUL samples. We believe using IUL for early detection of HGSC requires optimization, and current strategies should focus on prevention until early detection strategies improve.

KW - Early detection

KW - Intrauterine lavage

KW - Ovarian cancer

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ER -

Uterine washings as a novel method for early detection of ovarian cancer: Trials and tribulations

Abstract

Bibliographical note

Keywords

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