Utilization of the “complex method” to optimize a fermentation process

The complex method has been applied to maximize the profit of a fermentation process. The optimization method is based on sequential search technique and may be implemented in most process which can be stimulated. Utilizing the derived optimal value of the controllable variables (the concentration of glucose, soybean meal, phosphate, and salt; agitation and aeration rates; inoculum size; and fermentation duration) resulted in a substantial profit increase.