TY - JOUR
T1 - Vaccination with E. coli Recombinant Empty Viral Particles of Infectious Bursal Disease Virus (IBDV) Confer Protection
AU - Rogel, Arie
AU - Benvenisti, Luna
AU - Sela, Ilan
AU - Edelbaum, Orit
AU - Tanne, Edna
AU - Shachar, Yehoshua
AU - Zanberg, Yehuda
AU - Gontmakher, Tanya
AU - Khayat, Eli
AU - Stram, Yehuda
PY - 2003
Y1 - 2003
N2 - The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector pET21a under the T7 promoter, viral proteins were expressed and processed as demonstrated by Western blot analysis. Virus-like particles could be visualized by immuno-electron microscopy in IPTG-induced cells suggesting that viral assembly can take place in E. coli. Induction of anti-IBDV antibodies was detected in chickens immunized with purified recombinant IBDV by intra muscular (i.m.) injection. Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV.
AB - The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector pET21a under the T7 promoter, viral proteins were expressed and processed as demonstrated by Western blot analysis. Virus-like particles could be visualized by immuno-electron microscopy in IPTG-induced cells suggesting that viral assembly can take place in E. coli. Induction of anti-IBDV antibodies was detected in chickens immunized with purified recombinant IBDV by intra muscular (i.m.) injection. Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV.
KW - Empty particles
KW - Infectious bursal disease (IBDV)
KW - LA-RT-PCR
KW - Recombinant vaccines
UR - http://www.scopus.com/inward/record.url?scp=0141889729&partnerID=8YFLogxK
U2 - 10.1023/A:1025780611356
DO - 10.1023/A:1025780611356
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C2 - 14501195
AN - SCOPUS:0141889729
SN - 0920-8569
VL - 27
SP - 169
EP - 175
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -