Vaccinia virus directed RNA and protein synthesis in the presence of rifampicin

Bernard Moss*, Ehud Katz, Edith N. Rosenblum

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The effects of rifampicin, an inhibitor of bacterial DNA transcription, on the formation of infectious vaccinia virus and viral particles, RNA and proteins were studied under single step growth conditions. Although virion formation was inhibited by more than 99%, the synthesis of early and late viral directed RNA species, as characterized by their sedimentation properties, was unaffected. Rifampicin did not prevent the synthesis of early and late viral proteins identified by immunodiffusion, disc gel electrophoresis and disc gel immunoelectrophoresis. The drug also did not inhibit the in-vitro activity of the virion associated RNA polymerase.

Original languageEnglish
Pages (from-to)858-865
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume36
Issue number5
DOIs
StatePublished - 22 Aug 1969
Externally publishedYes

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