Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

S. M. Bergmann; Q. Wang; W. Zeng; Y. Li; Y. Wang; M. Matras; M. Reichert; D. Fichtner; M. Lenk; T. Morin; N. J. Olesen; H. F. Skall; P. Y. Lee; S. Zheng; S. Monaghan; S. Reiche; W. Fuchs; M. Kotler; K. Way; G. Bräuer; K. Böttcher; A. Kappe; J. Kielpinska

doi:10.1111/jfd.12621

Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

S. M. Bergmann^*, Q. Wang, W. Zeng, Y. Li, Y. Wang, M. Matras, M. Reichert, D. Fichtner, M. Lenk, T. Morin, N. J. Olesen, H. F. Skall, P. Y. Lee, S. Zheng, S. Monaghan, S. Reiche, W. Fuchs, M. Kotler, K. Way, G. BräuerK. Böttcher, A. Kappe, J. Kielpinska

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

Original language	English
Pages (from-to)	1511-1527
Number of pages	17
Journal	Journal of Fish Diseases
Volume	40
Issue number	11
DOIs	https://doi.org/10.1111/jfd.12621
State	Published - Nov 2017

Bibliographical note

Publisher Copyright:
© 2017 John Wiley & Sons Ltd

Keywords

Carp and koi sera
Enzyme-linked immunosorbent assay
Koi herpesvirus
Koi herpesvirus antibodies

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/jfd.12621

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Bergmann, S. M., Wang, Q., Zeng, W., Li, Y., Wang, Y., Matras, M., Reichert, M., Fichtner, D., Lenk, M., Morin, T., Olesen, N. J., Skall, H. F., Lee, P. Y., Zheng, S., Monaghan, S., Reiche, S., Fuchs, W., Kotler, M., Way, K., ... Kielpinska, J. (2017). Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Journal of Fish Diseases, 40(11), 1511-1527. https://doi.org/10.1111/jfd.12621

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abstract = "Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.",

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author = "Bergmann, {S. M.} and Q. Wang and W. Zeng and Y. Li and Y. Wang and M. Matras and M. Reichert and D. Fichtner and M. Lenk and T. Morin and Olesen, {N. J.} and Skall, {H. F.} and Lee, {P. Y.} and S. Zheng and S. Monaghan and S. Reiche and W. Fuchs and M. Kotler and K. Way and G. Br{\"a}uer and K. B{\"o}ttcher and A. Kappe and J. Kielpinska",

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year = "2017",

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Bergmann, SM, Wang, Q, Zeng, W, Li, Y, Wang, Y, Matras, M, Reichert, M, Fichtner, D, Lenk, M, Morin, T, Olesen, NJ, Skall, HF, Lee, PY, Zheng, S, Monaghan, S, Reiche, S, Fuchs, W, Kotler, M, Way, K, Bräuer, G, Böttcher, K, Kappe, A & Kielpinska, J 2017, 'Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)', Journal of Fish Diseases, vol. 40, no. 11, pp. 1511-1527. https://doi.org/10.1111/jfd.12621

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T1 - Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

AU - Bergmann, S. M.

AU - Wang, Q.

AU - Zeng, W.

AU - Li, Y.

AU - Wang, Y.

AU - Matras, M.

AU - Reichert, M.

AU - Fichtner, D.

AU - Lenk, M.

AU - Morin, T.

AU - Olesen, N. J.

AU - Skall, H. F.

AU - Lee, P. Y.

AU - Zheng, S.

AU - Monaghan, S.

AU - Reiche, S.

AU - Fuchs, W.

AU - Kotler, M.

AU - Way, K.

AU - Bräuer, G.

AU - Böttcher, K.

AU - Kappe, A.

AU - Kielpinska, J.

PY - 2017/11

Y1 - 2017/11

N2 - Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

AB - Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

KW - Carp and koi sera

KW - Enzyme-linked immunosorbent assay

KW - Koi herpesvirus

KW - Koi herpesvirus antibodies

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JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

IS - 11

ER -

Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

Abstract

Bibliographical note

Keywords

UN SDGs

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